| 凝结芽孢杆菌TQ33产抗疫霉物质特性研究 |
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| 引用本文： | 肖瑞峰,闫艳华,宗志友,王海宽.凝结芽孢杆菌TQ33产抗疫霉物质特性研究[J].天津科技大学学报,2011,26(6):1-6. |
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| 作者姓名： | 肖瑞峰 闫艳华 宗志友 王海宽 |
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| 作者单位： | 工业发酵微生物教育部重点实验室,天津科技大学生物工程学院,天津300457 |
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| 摘 要： | 凝结芽孢杆菌TQ33是从脱脂奶粉中分离出来的,发现其发酵液中活性代谢产物对甜瓜疫霉菌(Phytophthora drechsleri Tucker)、灰葡萄孢霉菌(Botrytis cinerea)、番茄早疫病菌(Alternaria solani)、甜瓜枯萎病菌(Fusarium ox-ysporum)、苹果炭疽病菌(Glomerella cingulate)植物致病真菌有很好的抑制作用,其中对甜瓜疫霉菌具有较强的抑制作用,同时对抗甜瓜疫霉物质特性进行了研究,将具有抑菌活性的发酵液分别经过调节pH、加热、蛋白酶处理后发现,凝结芽孢杆菌TQ33发酵液具有一定的热稳定性;在pH,5～6时具有最大抑菌活性,发酵液经胰蛋白酶、中性蛋白、蛋白酶K处理后失去部分抑菌活性,初步分离纯化实验表明其中一种抗菌物质可能是苯乳酸,这表明凝结芽孢杆菌TQ33发酵液中可能含有苯乳酸,以及其他蛋白类抑菌物质.
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| 关 键 词： | 植物致病真菌 抗真菌活性 凝结芽孢杆菌TQ33 甜瓜疫霉菌 |
Activity against Phytophthora drechsleri Tucker of Bacillus coagulans TQ33 and the Properties of Bioactive Substance |
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| XIAO Rui-feng,YAN Yan-hua,ZONG Zhi-you,WANG Hai-kuan.Activity against Phytophthora drechsleri Tucker of Bacillus coagulans TQ33 and the Properties of Bioactive Substance[J].Journal of Tianjin University of Science & Technology,2011,26(6):1-6. |
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| Authors: | XIAO Rui-feng YAN Yan-hua ZONG Zhi-you WANG Hai-kuan |
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| Institution: | XIAO Rui-feng,YAN Yan-hua,ZONG Zhi-you,WANG Hai-kuan(Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education,College of Biotechnology,Tianjin University of Science & Technology,Tianjin 300457,China) |
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| Abstract: | Antifungal effects on plant pathogenic fungi growth and characteristics of the active antifungal substances were produced by Bacillus coagulans TQ33,which isolated from the skimmed milk powder.B.coagulans TQ33 has a broad anti-fungal spectrum including Phytophthora drechsleri Tucker,Fusarium oxysporum,Botrytis cinerea,Alternaria solani and Glomerella cingulate,particularly against P.drechsleri Tucker.The effect of pHt,emperature and protease on the antifungal activity of B.coagulans TQ33 was determined.The ... |
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| Keywords: | plant pathogenic fungi antifungal activity Bacillus coagulans TQ33 Phytophthora drechsleri Tucker |
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