| 鸡胚背根神经节的摘取方法及培养条件的改进优化 |
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| 引用本文： | 单莉娅,张云华,朱美意,朱井玲,汪海燕,黄瑾. 鸡胚背根神经节的摘取方法及培养条件的改进优化[J]. 石河子大学学报(自然科学版), 2013, 0(5): 612-617 |
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| 作者姓名： | 单莉娅 张云华 朱美意 朱井玲 汪海燕 黄瑾 |
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| 作者单位： | 石河子大学医学院生物化学教研室／新疆地方与民族高发病教育部重点实验室,石河子832002 |
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| 基金项目： | 石河子大学自然科学研究项目（ZRKX2010ZD03） |
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| 摘 要： | 探讨摘取鸡胚DRG的有效方法，并对培养条件进行优化，为神经营养因子突起鉴定奠定基础。根据鸡胚DRG的生长状态和解剖特点，采用分部摘取法摘取鸡胚DRG，利用NGF对不同部位的DRG进行鉴定，同时通过不同血清浓度、不同培养基及不同观察时间鸡胚DRG神经突起的生长状态，对培养条件进行优化。结果显示分部摘取法摘取鸡胚DRG提高了实验的可操作性和可重复性；综荐骨部的鸡胚DRG为实验最佳取材部位；无血清RPMI-1640培养基为最适神经突起生长鉴定培养基。
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| 关 键 词： | 鸡胚背根神经节 神经营养因子 生物活性 |
The Improvement and Optimization of the Method of Dissecting Chicken Embryo Dorsal Root Ganglion and its Culture Conditions |
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| SHAN Liya,ZHANG Yunhua,ZHU Meiyi,ZHU Jingling,WANG Haiyan,HUANG Jin. The Improvement and Optimization of the Method of Dissecting Chicken Embryo Dorsal Root Ganglion and its Culture Conditions[J]. Journal of Shihezi University(Natural Science), 2013, 0(5): 612-617 |
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| Authors: | SHAN Liya ZHANG Yunhua ZHU Meiyi ZHU Jingling WANG Haiyan HUANG Jin |
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| Affiliation: | (Key Laboratory of Xinjiang Endemic and Ethnic Disease of Ministry of Education/ Department of Biochemistry, School of Medicine/, Shihezi University, Shihezi 832002,China) |
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| Abstract: | Objective：To study the effective method of dissecting chicken embryo DRG and optimize its culture conditions so as to lay a foundation for neurotrophic factor neurite outgrowth identification. Method：According to the growth status and anatomical features of chicken embryo DRG, by separate dissecting method, we dissected chicken embryo DRG, and identified different parts of DRG with NGF;at the same time,by analyzing the effect of different concentration of serum,different medium and dif- ferent observation time on the neurite outgrowth of chicken embryo DRG, we optimized the culture conditions. Result：Dissec- ting chicken embryo DRG with separate dissecting method improves the maneuverability and repeatability of the experiment; the optimum material of chicken embryo DRG for experiment is the part of synsacrum;the optimum medium for neurite outgrowth identification is serum-free RPMI-1640 medium. |
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| Keywords: | dorsal root ganglia of chicken embryo neurotrophin biological activity |
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