苦杏仁苷与人血清白蛋白相互作用的荧光光谱研究 |
| |
引用本文: | 何丽君;霍彩霞;徐飞;李生英;魏云霞;程小丽. 苦杏仁苷与人血清白蛋白相互作用的荧光光谱研究[J]. 甘肃教育学院学报(自然科学版), 2013, 0(5): 55-57 |
| |
作者姓名: | 何丽君 霍彩霞 徐飞 李生英 魏云霞 程小丽 |
| |
作者单位: | 兰州城市学院化学与环境科学学院 |
| |
基金项目: | 甘肃省城市发展研究院科研项目(2012-GSCFY-KJ04) |
| |
摘 要: | 应用荧光光谱法,研究了苦杏仁苷与人血清白蛋白(HSA)在pH=7.40的Tris-HCl缓冲溶液中相互作用的情况.研究表明,苦杏仁苷通过静态机理猝灭了HSA的内源荧光.并计算得到苦杏仁苷与HSA的结合常数和结合位点数.通过热力学数据,推断出苦杏仁苷与人血清白蛋白之间主要靠氢键和范德华力结合.同时采用同步荧光技术考察了苦杏仁苷对HSA构象的影响.
|
关 键 词: | 苦杏仁苷 人血清白蛋白 相互作用 荧光光谱 |
Study on the Interaction between Amygdalin and Human Serum Albumin by Fluorescence Spectroscopy |
| |
Affiliation: | HE Li-jun;HUO Cai-xia;XU Fei;LI Sheng-ying;WEI Yun-xia;CHENG Xiao-li;School of Chemistry and Environmental Science,Lanzhou City University; |
| |
Abstract: | The interaction between amygdalin and human serum albumin(HSA)in buffer solution(pH =7.40)were studied by fluorescence spectroscopy.It was found that amygdalin quenched the fluorescence of HSA via a static quenching process.The binding constant and number of binding sites were found.Values of thermodynamic parameters were calculated.These dates indicated that hydrogen bonds and van der Waals force played a major role in the binding of amygdalin and HSA.Using synchronous fluorescence spectroscopy,the effect of amygdalin on the configuration of HSA was studied. |
| |
Keywords: | amygdalin human serum albumin interaction fluorescence spectroscopy |
本文献已被 维普 等数据库收录! |