| 棉花DELLA蛋白GhGAl4a基因在拟南芥中功能初步分析 |
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| 引用本文： | 吕西洋,崔百明,段琛,陈英杰,黄先忠,郑银英. 棉花DELLA蛋白GhGAl4a基因在拟南芥中功能初步分析[J]. 石河子大学学报(自然科学版), 2013, 0(5): 529-535 |
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| 作者姓名： | 吕西洋 崔百明 段琛 陈英杰 黄先忠 郑银英 |
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| 作者单位： | [1]石河子大学生命科学学院／石河子大学农业生物技术重点实验室,石河子832003 [2]国际关系学院,北京10091 |
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| 基金项目： | 转基因生物新品种培育科技重大专项（2011ZX08005-002）,转基因生物新品种培育重大专项（2009ZX08005-027B） |
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| 摘 要： | 为了探究棉花DELLA蛋白基因GhGAI4a在拟南芥中的功能，构建植物表达载体pBP35S：GhGAI4a和pBP35S：Ghgai4a，利用农杆菌介导的花滴法将其转入Col野生型拟南芥。结果显示，2个载体各自获得6个独立的转基因拟南芥纯合株系。分别统计48—96h种子萌发率，测量生长7d后幼苗的主根长度，与非转基因植株相比，过量表达GhGAI4a、Ghgai4a对拟南芥种子萌发及主根生长具有明显的抑制作用。1μmol／LGA处理转基因植株，GhGAI4a转基因植株主根长和萌发率均增大，而Ghgai4a转基因植株主根长度和萌发率均无显著变化。
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| 关 键 词： | 棉花 GhGAI4a基因 DELLA蛋白 赤霉素 |
Functional Analysis of Cotton DELLA Protein GhGAI4a Gene in Arabidopsis thaliana |
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| LU Xiyang,CUI Baiming,DUAN Shen,CHEN Yingjie,HUANG Xianzhong,ZHENG Yinying. Functional Analysis of Cotton DELLA Protein GhGAI4a Gene in Arabidopsis thaliana[J]. Journal of Shihezi University(Natural Science), 2013, 0(5): 529-535 |
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| Authors: | LU Xiyang CUI Baiming DUAN Shen CHEN Yingjie HUANG Xianzhong ZHENG Yinying |
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| Affiliation: | 1 Key Laboratory of Agrobiotechnology of Shihezi University/College of Life Sciences, Shihezi University, Shihezi 832003, China; 2 University of International Relations, Beijing 100091, China) |
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| Abstract: | To analyze the function of cotton DELLA protein GhGAI4a gene in Arabidopsis thaliana, we constructed the plant expression vector pBP35S ： GhGAI4a and pBP35S ： Ghgai4a and converted them into Arabidopsis thaliana Col with the method of Agrobacterium-mediated inflorescence infection. Then through the kanamycin screening, the test of polymerase chain reaction （PCR） technology and the semi-quantitative RT-PCR analysis, we obtained six separate transgenic Arabidopsis homozygous from each of the two vectors. We made statistics about the 48-96 h seed germination rate,and measured the taproot length of the 7 d seedling. Compared with non-transgenetic plants,GhGAI4a and Ghgai4a have obvious inhibitory effect on seed germination and root growth. Meanwhile, through 1 μmol/L GA treatment, the taproot length and germination rate of GhGAI4a transgenic plants increased while the Ghgai4a transgenic plants did not change obviously. |
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| Keywords: | cotton GhGAI4a gene DELLA protein gibberellin |
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