PRMT5 suppresses DR4-mediated CCL20 release via NF-κB pathway |
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Authors: | DongSheng Wang Dan Liu Jing Gao Min Liu ShiLian Liu YanXin Liu DeXian Zheng |
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Institution: | 1. National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100005, China 2. Department of Laboratory Medicine, Affiliated Hospital of North Sichuan Medical College, Nanchong, 637000, China
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Abstract: | Construct expression vectors of pCMV-DR4-HA and pCMV-PRMT5-Flag,and transfect them into HEK293 cells to identify the interaction between TRAIL-R1 and PRMT5 and the molecular mechanism underlying DR4-mediated inhibition of chemokine CCL20 release via TRAIL receptor 1(DR4).Inflammatory cytokine was detected by RT-PCR and ELISA after TRAIL-R1 and/or PRMT5 transfection,respectively.NF-κB activity was detected by Dual Luciferase Reporter Gene Assay.ERK1/2 phosphorylation was analyzed by Western blot.PRMT5 could inhibit DR4-activated NF-κB activity and ERK1/2 phosphorylation.PRMT5 could inhibit NF-κB activition,ERK1/2 phosphorylation as well as CCL20 secretion via binding with DR4 in HEK293 cell,suggesting that PRMT5 may involve in DR4 dependent immune regulation. |
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Keywords: | TRAIL PRMT5 cytokine CCL20 |
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