Effect of PKC pathway on G1/S progression control in HeLa cells

The effect of PKC activity on G₁/S progression in HeLa cells has been studied. The result shows that (i) PKC activity alteration in G₁ phase affects G₁/S progression in HeLa cells. It has been observed that G₁/S progression is stimulated by PKC agonist TPA and inhibited by PKC inhibitor GF-109203X. (ii) The expression ofc-myc andc-jun is stimulated by TPA and inhibited by GF-109203X treatment in early G₁ phase. (iii) During G₁/S progression, the expression of CyclinD₁ is stimulated by TPA treatment and inhibited by GF-109203X treatment. There is no effect on the expression of CDK4. It is likely that PKC pathway regulates G₁/S progression through regulating the expression of some early response genes and engine molecules in HeLa cells.