不同剂量甾体激素对大鼠动情周期及非动情周期子宫中MMP-2活性的影响

本实验采用酵谱(Zymography)法研究了不同剂量的甾体激素对动情周期及切除双侧卵巢的大鼠子宫中MMP—2活性的影响,结果显示,4种不同剂量的雌二醇(0.05mg,0.1mg,0.3mg0.5mg)对动情后期及切除双侧卵巢后的大鼠子宫中MMP—2均有促进作用,其中67kD,66kD和64kL MMP—2的活性变化与雌二醇剂量变化呈正比关系.动情后期大鼠子宫中MMP—2活性.4种不同剂量(0.2mg,0.4mg,0.6mg和0.8mg)的孕酮对处于动情前期大鼠子宫中MMP—2活性没有显著作用,但是对切除双侧卵巢14天的大鼠子宫中MMP—2活性有明显抑制作用,其活性变化与孕酮剂量成反比.结果揭示:大鼠体内甾体激素分泌量的变化导致子宫MMP—2活性的变化,甾体激素对MMP—2活性的影响与子宫内膜的生理状态密切相关。

EFFECTS OF DIFFERENT DOSAGE STREROID HORMONES ON MMP-2 ACTIVITY OF RAT UTERUS IN ESTROUS CYCLE AND NON-ESTROUS CYCLE

This experiment, using Zymograhy method, studied the effects of different dosages of streroid hormones on MMP-2 activity in rat uterus during estrous cycle. The results showed that four kinds of different dosage estradiol(0.05mg,0.lmg,0.3mg,0. 5mg)increased MMP-2 activity in rat uterus during estrous cycle and ovariectomied. A-mong them,the change of MMP-2 activity of 67kD,66kD,and 64kD was in direct proportion to the change of the estradiol dosage. MMP-2 activity of rat uterus in metoestrus is higher than that of the ovariectomied. The four kinds of different dosage progesterone (0. 2mg,0. 4mg 0. 6mg 0. 8mg)had no significant effects on the MMP-2 activity of rat uterus during the proestrus, but they inhibited significantly the MMP-2 activity of rat uterus which was ovariectomied 14 days,and their change of activity was in inverse proportion to the dosage of progesterone. The results also indicated that the change of secretion amount of streroid hormones in rat brought about the change of MMP-2 activity and the effects of streroid hormones on MMP-2 activity are closely related to the state of internal membrane of the uterus.