| 近海海洋真菌生物转化(+)-柠檬烯 |
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| 引用本文： | 杨道茂,陈煌.近海海洋真菌生物转化(+)-柠檬烯[J].华侨大学学报(自然科学版),2020,41(1):67-71. |
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| 作者姓名： | 杨道茂 陈煌 |
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| 作者单位： | 1. 华侨大学 化工学院, 福建 厦门 361021;2. 厦门万泰沧海生物技术有限公司, 福建 厦门 361022 |
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| 基金项目： | 华侨大学高层次人才科研启动基金 |
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| 摘 要： | 以厦门近海海域分离到的30株海洋真菌为出发菌株,通过液体发酵法考察其生物转化(+)-柠檬烯的能力,采用气相色谱-质谱(GC-MS)技术结合NIST数据库对其代谢产物进行化合物分析.结果表明:23,27,36,41号真菌具有生物转化柠檬烯的能力,其主要代谢产物暂定为异辣薄荷烯酮、波斯菊萜、1,3,8-孟三烯、香芹醇等;在23,36号真菌的代谢产物中,异辣薄荷烯酮为唯一主要产物,具有进一步研究开发的价值.
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| 关 键 词： | (+)-柠檬烯 海洋真菌 生物转化 液体发酵法 异辣薄荷酮 |
Biotransformation of(+)-Limoneneby Offshore Marine Fungi |
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| YANG Daomao,CHEN Huang.Biotransformation of(+)-Limoneneby Offshore Marine Fungi[J].Journal of Huaqiao University(Natural Science),2020,41(1):67-71. |
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| Authors: | YANG Daomao CHEN Huang |
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| Institution: | 1. College of Chemical Engineering, Huaqiao University, Xiamen 361021, China; 2. Xiamen Innovax Biotech Limited Company, Xiamen 361022, China |
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| Abstract: | Thirty marine fungi isolated from Xiamen City offshore district were used as starting strains to investigate their ability to biotransform(+)-limonene by liquid fermentation method, and their metabolites were analyzed by gas chromatography-mass spectrometry(GC-MS)combined with NIST database. The results showed that strain 23, 27, 36 and 41 had the biotransformation ability of limonene. The main metabolites were tentatively identified as isopiperitenone, cosmene, 1,3,8-menthatriene, carveol, etc. The isopiperitenone was the only major product among metabolites produced from strain 23 and 36, which is valuable for further research and exploitation. |
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| Keywords: | (+)-limonene marine fungi biotransformation liquid fermentation method isopiperitenone |
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