| 胎牛成纤维细胞的分离培养 |
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| 引用本文： | 尹明,章轶哲,多曙光.胎牛成纤维细胞的分离培养[J].实验动物科学,2010,27(3):1-6. |
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| 作者姓名： | 尹明 章轶哲 多曙光 |
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| 作者单位： | 1. 北京市维通达生物技术有限公司,北京,100107
2. 北京大学生命科学学院,北京,100871 |
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| 摘 要： | 应用组织块培养法从胎牛耳部分离培养了胎牛成纤维细胞。应用PCR方法鉴别了细胞的性别。选择雌性成纤维细胞,进行了形态观察、生长曲线测定、染色体分析。结果表明,分离培养的细胞具有正常的大小、形态、分裂增殖特性和染色体数目。胎牛成纤维细胞的培养方法的建立以及其相关的生物学特性的分析,有利于给同种和异种体细胞克隆牛研究提供形态良好、染色体数目正常的供体细胞,同时也为体细胞转基因等其他领域的研究提供了基础条件。
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| 关 键 词： | 胎牛成纤维细胞 分离培养 性别鉴定 染色体分析 |
| 收稿时间： | 2010-06-28 |
Isolation, Culture and Related Biological Characteristics of Bovine Fetal Fibroblasts |
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| YIN Ming,ZHANG Yi-zhe,DUO Shu-guang.Isolation, Culture and Related Biological Characteristics of Bovine Fetal Fibroblasts[J].Shiyan Dongwu Kexue,2010,27(3):1-6. |
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| Authors: | YIN Ming ZHANG Yi-zhe DUO Shu-guang |
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| Institution: | 1. Beijing VitalStar Biotechnology Co. Ltd. Beijing 100107,China) (2. College of Life Sciences,Peking University,Beijing 100871,China) |
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| Abstract: | The bovine fetal otic fibroblast cells were isolated using tissue bulk attachment. The gender of fetus was identified using PCR. The fibroblast cells from female fetus were chosen for further morphologic observation,growth curve and chromosome analyzing. The results indicated that the cells had normal morphology,multiplication characteristics and chromosome number. The establishment of culture method and related biological characterization analysis of bovine fetal fibroblast provided not only a ready supply of somatic cells for the intraspecies and interspecies cloning of macaque but also fundamental conditions for somatic transgene and other related fields. |
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| Keywords: | Bovine fetal fibroblast Isolation and culture Sex identification Chromosome analysis |
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