小球藻(Chlorella vulgaris)产油脂最佳培养条件的筛选

以低温作为环境条件诱导小球藻(Chlorella vulgaris)产脂,采用正交试验的方法来研究不同水平下Mn,K,Mg和S各元素对小球藻生长及脂肪产量的影响.本实验C N-1比为41.2:1,pH值范围为7.4~7.5之间,培养温度为15℃,P浓度为0.2~0.4 mmol.L-1,Fe浓度为0.04 mmol.L-1,以自养生长型式培养,而培养基成份根据BG11培养基作为基础进行改良.根据实验结果显示,脂肪产量最高为实验编号10(64.59 mg(100 mL)-1,64.59%),即锰及镁在一定程度上能促进小球藻的生长,且脂肪产率更高.

Section of the Best Culture Conditions in Chlorella Fatty Acid Croduction

By low temperature inducing chlorella vularis to produce fatty acid,orthogonal test is used to study the combination of different levels elements Mn,K,Mg and S to affect the fatty acid yields.In the experiment,C N-1 ratio is 41.2∶1,pH values range between 7.4 to 7.5,temperature is 15 ℃,concentration of P is 0.2~0.4 mmol·L-1,concentration of Fe is 0.04 mmol-1,autotrophic culture is used between the research,and the medium is improved which is based on BG11 medium.The highest fatty acid yield is experiment No.10(64.59 mg(100 mL)-1,64.59%).According to the results,Mn could increase a certain extent in the fatty acid yield.