VIP receptors and control of short circuit current in the human intestinal clonal cell line Cl.19A |
| |
| Authors: | C Rouyer-Fessard C Augeron E Grasset J J Maoret C L Laboisse M Laburthe |
| |
| Institution: | Unité de Recherche sur la Différenciation et la Neuroendocrinologie des Cellules Digestives, INSERM U178, Villejuif, France. |
| |
| Abstract: | At the maximally effective concentration of 10 nM, VIP induced a marked (12.5-fold stimulation above basal), and sustained increase in short circuit current in the human intestinal epithelial cell line Cl.19A grown on permeable filters and placed in Ussing chambers. Half-maximal increase of Isc was observed for 0.1 nM VIP. This was well correlated with the VIP-stimulated adenylate cyclase activity (ED50:0.07 nM). Binding studies using 125I-VIP indicated that Cl.19A cells express a peptide-specific VIP receptor with a dissociation constant of 0.07 nM. Covalent labeling of receptors followed by SDS-PAGE analysis of membrane proteins resulted in the identification of a 63,000 dalton binding protein in Cl.19A cells. |
| |
| Keywords: | |
|
|
