小鼠胚肝基质细胞在体外培养中的动态演化

胚肝基质细胞是研究胚肝造血调控的重要工具，本实验对小鼠胚肝基质细胞在体外培养过程中的生长、细胞类型组成及其组成比例的演变过程进行了观察．实验显示小鼠原代培养初期基质细胞是由平滑肌样上皮细胞、巨噬细胞以及成纤维样细胞、树突状细胞、内皮细胞构成的，经过多次传代后，巨噬细胞等类型的细胞逐渐丢失，平滑肌样上皮细胞和成纤维样细胞成为主体，表明体外培养的小鼠胚肝基质细胞在4代以内都能较真实的反映体内造血微环境的构成，适用于胚肝造血研究．此为进一步研究胚肝基质细胞在胚胎期造血过程中的作用奠定了工作基础．

Dynamic evolvement of murine fetal liver stromal cells in vitro

The fetal liver stromal cell is considered to be the important measure to investgate the mechanisms that regulate the hematopoiesis. The present work aimed at the growth, constitution, and proportion evolvement of the murine fetal liver stromal cells in vitro. The results show that murine fetal liver stromal cells are constituted of vascular smooth muscle-like epithelial cells, macrophages, fibroblast cells, dendritic cells and endothelial cells. After repetitious subculture, vascular smooth muscle-like epithelial cell and fibroblast cell become the major components accompanied with the decrease of other cells. These indicate only the fetal liver stromal cells before F4 subculture can reflect the hematopoietic microenvironment in vivo. These results will be useful to further analyze the mechanism of fetal hematopoietic regulation.