慢性氟中毒对大鼠基础生长的影响及金属离子的拮抗作用

目的 通过动物实验,研究慢性氟中毒对大鼠生长的影响,以及钙、镁和铝离子对慢性氟中毒的拮抗作用,为研究地氟病的分子机制及其治疗提供基础数据.方法 断奶1周的24只雄性SD大鼠,随机分成4个实验组,分别为对照组、高氟组、氟＋镁＋铝拮抗组和氟＋钙拮抗组.全群饲喂3个月,每隔两天称量大鼠体质量、摄食量和饮水量.处死前3d,每天留取24 h尿液,测定尿氟含量.结果 1）与对照组相比,高氟组体质量增长速度明显减缓,加钙拮抗组体质量增长速度比对照组快,也比高氟组快；2）高氟组的尿氟含量高于其他组,加镁铝拮抗组和加钙拮抗组比高氟组低,但仍高于对照组；3）各组摄食量和饮水量无明显差异.结论 慢性氟中毒会减缓动物个体的生长速度,而在饮水中添加镁、铝或钙离子能够起到一定的拮抗作用,钙离子拮抗作用更为明显.

Effects of Chronic Fluorosis on Rat Growth and the Antagonism of Mg2+ ,Al3+ and Ca2+

Objective To study the effects of chronic fluorosis on the growth of rate and the antagonism of Mg2＋,Al3＋ and Ca2＋ to fluorine toxicity so as to provide the data for the researches on the molecular mechanism and the treatment of endemic fluorosis.Method 24 the first week postweaning male SD rats were randomly divided into 4 groups （n =6 in each group）,namely the control group （group 1）,fluoridating group （group 2）,fluorine ＋ Mg2 ＋ ＋ Al3 ＋ group （group 3） and fluorine ＋ Ca2 ＋ group （group 4）.The water added with fluorine,with fluorine ＋ Mg2 ＋ ＋ Al3 ＋ and with fluorine ＋ Ca2 ＋ was respectively given to the rats in the different groups for three months.The body weight,food intake and the amount of water drinking were measured and recorded every two days,and the 24-hour urine was collected daily to determine the fluorine content in the urine during 3 days before sacrifice.Result The speed of weight gain in group 4 was significantly higher than that in group 1,and the speed of weight gain in group 2 and group 3 was significantly lower than that in group 1.Urine fluorine content of group 2 was higher than that of the other 3 groups,while that of group 3 and group 4 was lower than group 2 but higher than group 1.There was no significant difference in food intake and amount of water drinking among groups.Conclusion The chronic fluorosis could decelerate the growth rate,while adding Mg2＋,Al3＋ or Ca2＋ into the drinking water,especially Ca2＋,may antagonize this effect.