| 乳胞素诱导帕金森病大鼠模型行为学及病理学观察 |
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| 引用本文： | 朱少平,胡利,鲍波.乳胞素诱导帕金森病大鼠模型行为学及病理学观察[J].实验动物科学,2013,30(4):26-30. |
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| 作者姓名： | 朱少平 胡利 鲍波 |
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| 作者单位： | 1. 广东医学院实验动物中心实验动物学教研室,湛江524023;广东医学院病理生理学教研室,湛江524023
2. 广东医学院组织学与胚胎学教研室,湛江,524023 |
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| 基金项目： | 广东省科技计划项目(项目编号：2010B060500015) |
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| 摘 要： | 目的用乳胞素诱导SD大鼠建立帕金森病(Parkinson’s Disease,PD)模型,并进行行为学及病理学观察。方法乳胞素/DMSO(8μg/2μL)定向注射模型组大鼠左侧脑黑质内,DMSO组注射DMSO。于手术后第6、13和20天采用阿朴吗啡旋转实验检测行为学改变,用免疫组织化学法检测脑组织切片,进行TH阳性细胞计数及观察α突触核蛋白(α-synuclein,α-syn)聚集情况。结果模型组中SD大鼠向右旋转速度大于7 r/min;脑组织切片中可见α-syn聚集。与对照组相比,模型大鼠脑组织切片中TH阳性细胞数显著减少(P<0.05),模型组第10天处死大鼠与第20天处死大鼠相比,TH阳性细胞数差异具有统计学意义(P<0.05)。结论乳胞素单侧黑质内注射可以诱导大鼠PD模型。
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| 关 键 词： | 帕金森病 乳胞素 动物模型 SD大鼠 |
An Observation on Ethological and Pathological Changes in Rat of Parkinson's Disease Induced by Lactacystin |
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| ZHU Shao-ping
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HU Li
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BAO Bo.An Observation on Ethological and Pathological Changes in Rat of Parkinson's Disease Induced by Lactacystin[J].Shiyan Dongwu Kexue,2013,30(4):26-30. |
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| Authors: | ZHU Shao-ping HU Li BAO Bo |
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| Institution: | 1.Department of Laboratory Animal, Guangdong medical College, Zhanjiang 524023, China; Department of Pathophysiology , Guangdong Medical College, Zhanjiang 524023, China; 2.Department of Histology and Embryology, Guangdong Medical College, Zhanjiang 524023, China;) |
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| Abstract: | Objective To observe the ethological and pathological changes of the rats of parkinson＇s disease induced by injection of lactacystin into the substantia nigra.Method Lactacystin/DMSO （8 μg/2 μL） was stereotacticly injected into the left substantia nigra of model group,while the DMSO was injected into the left substantia nigra into DMSO group.Apomorphineinduced rotation test was performed on day 6,13 and 20 after the injection.Immunohistochemical staining was conducted to test the aggregation of α-synuclein and the number of TH （tyrosine hydroxylase） positive neurons.Result The rats of model group revolved rightward and the frequency exceed 7 r/min.Aggregation of α-synuclein was revealed in the paraffin section of substantia nigra of model rats and the number of TH-positive neurons were significantly reduced.Conclusion Rats model of PD can be established by stereotactic injection of lactacystin into the substantia nigra. |
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| Keywords: | Parkinson's disease lactacystin animal model SD rat |
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