包埋脱水法冰冻保存3种饵料金藻的研究

用包埋脱水法冰冻保存绿色巴夫藻(Pavlova viridis)、湛江等鞭藻(Isochrysis zhanjiangensis)和球等鞭金藻(Isochrysis galbana 3011)3种海洋饵料金藻，探讨了胶球含水量和预培养对冰冻保存存活率的影响．结果表明：3种藻冰冻前胶球的最佳含水量不同，绿色巴夫藻为35％。湛江等鞭藻和球等鞭金藻都为30％；预培养后3种藻都在20％含水量获得最高存活率，其中绿色巴夫藻经2％PEG预培养后存活率提高了6％，效果好于5％PVP预培养．预培养对其他两种藻的存活率没有提高．

Cryopreservation of Three Golden Algae Used as Food in Mariculture by Encapsulation-dehydration

Three marine golden algae used as food, Pavlova viridis, Isochrysis zhanjiangensis and Isochrysis galbana 3011, were preserved in liquid nitrogen using encapsulation-dehydration. Some factors influencing cell viability, such as bead water content and preculture were studied. The results showed every alga had its optimum water content of beads before freezing, that of Pavlova viridis was 35%, that of Isodhrysis zhanjiangensis and Isochrysis galbana 3011 was 30%; after preculture, three algae obtained the highest viabilities at 20% water content, and the highest viability of Pavlova viridis was increased by 6% after the preculture of 2%PEG, which was better than that of 5% PVP. However, preculture did not increase the viability of the other two algae.