| 柿树胚乳培养及其再生植株染色体倍性变化的研究 |
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| 引用本文： | 庄东红,石田雅士.柿树胚乳培养及其再生植株染色体倍性变化的研究[J].汕头大学学报(自然科学版),1995,10(1):42-47. |
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| 作者姓名： | 庄东红 石田雅士 |
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| 作者单位： | 汕头大学生物学系，日本京都府立大学农学部 |
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| 摘 要： | 用ＭＳ＋ＢＡ５ｍｇ／Ｌ＋ＩＢＡ０．０１ｍｇ／Ｌ培养基培养柿树品种“黑柿”、“长良”的胚乳愈伤组织获再生植株．再生植株的根尖染色体数目为２ｎ＝９０或１８０，无发现存在２ｎ＝１３５的个体．而作为外植体的胚乳细胞检查为３ｎ＝１３５．表明愈伤组织细胞在培养过程中发生了染色体数量的变化．
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| 关 键 词： | 柿树 胚乳植株 染色体数目 |
Changes in chromosomal ploidy in regenerated plant obtained from cultured endosperm of Diospyros kaki |
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| Zhuang Donghong.Changes in chromosomal ploidy in regenerated plant obtained from cultured endosperm of Diospyros kaki[J].Journal of Shantou University(Natural Science Edition),1995,10(1):42-47. |
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| Authors: | Zhuang Donghong |
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| Abstract: | Endosperm of Japanese persimmon (Diospyros kaki L. ) cv. "Nagara", "Kurogaki" were cultured and some regenerated shoots were obtained from endosperm callus on the MS media with 5. 0mg/L BA and 0. 01mg/L IBA. The chromosome number of the root tip cell was examined. Results indicated that the chromosome numbers in regenerated plants are 2n= 180 or 2n= 90, but not 2n= 135 as had been found in original endosperm cells. It suggested that a change in ploidy had occurred in callus cells during culture. |
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| Keywords: | Diospyros kaki endosperm plant chromosome number |
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