| 基于RAPD标记的我国水青冈属植物的分类研究 |
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| 引用本文： | 吉成均,沈海花,方精云,谢长富,樊拥军. 基于RAPD标记的我国水青冈属植物的分类研究[J]. 北京大学学报(自然科学版), 2002, 38(6): 817-822. DOI: 10.3321/j.issn:0479-8023.2002.06.013 |
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| 作者姓名： | 吉成均 沈海花 方精云 谢长富 樊拥军 |
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| 作者单位： | 1. 北京大学城市与环境学系,北京大学地表过程分析模拟教育部重点实验室,北京,100871;2. 台湾大学植物学系,台北,106;3. 南开大学生命科学学院,天津,300071 |
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| 基金项目： | 国家自然科学基金;39830050,39970044; |
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| 摘 要： | 应用随机引物扩增多态性DNA(RAPD)方法,对我国水青冈属(Fagus L.)6种植物,即巴山水青冈(F.pashanica)、长柄水青冈(F.longipetiolata)、米心水青冈(F.engleriana)、亮叶水青冈(F.lucida)、台湾水青冈(F.hayatae)和浙江水青冈(F.hayatae var.zhejiangensis)进行基因组多态性分析,选用80个随机引物进行扩增,其中56个引物扩增出清晰可重复的条带,片段大小在250～3050 bp之间.采用UPGMA法对求出的遗传片段进行聚类分析.结果显示:台湾水青冈、巴山水青冈和浙江水青冈的遗传距离较近,表明它们可归并为一种.这与我国水青冈属的生态解剖学研究结果完全一致.根据结果将我国水青冈植物归并分为4种:长柄水青冈、米心水青冈、亮叶水青冈、台湾水青冈(包括巴山水青冈和浙江水青冈).
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| 关 键 词： | 水青冈 RAPD技术 遗传距离 |
RAPD Analyses of Fagus Species in China |
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| JI Chengjun SHEN Haihua FANG Jingyun. RAPD Analyses of Fagus Species in China[J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2002, 38(6): 817-822. DOI: 10.3321/j.issn:0479-8023.2002.06.013 |
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| Authors: | JI Chengjun SHEN Haihua FANG Jingyun |
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| Abstract: | Random amplified polymorphic DNA (RAPD) technique was applied to assess six Fagus species in China.Fifty-six of eighty tested arbitrary primers generated obvious bands with size ranging from 250 to 3*!050*!bp.An index of genetic similarity(F)was calculated and the value of (1-F) was used to evaluate genetic distances between species to construct dendrogram.The result suggested that three species,F.pashanica,F.hayaatae and F.hayatae var.zhejiangensis,had closer relationship and should be clustered into a single species.This was in good agreement with our morphological work.Based on RAPD markers,we suggested that Chinese beech species should be divided into four species,F.longipetiolata,F.engleriana,F.lucida and F.hayaatae(including F.pashanica and F.hayatae var.zhejiangensis). |
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| Keywords: | Fagus RAPD technique genetic distance |
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