A multiple-labelling method for cells using Au nanoparticles with different shapes |
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Authors: | Ke Zhang JianTao Feng QuanMei Sun Lin Jin Jing Li XiaoChun Wu Dong Han |
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Affiliation: | 15794. National Centre for Nanoscience and Technology, Chinese Academy of Sciences Key Laboratory of Standardisation and Measurement for Nanotechnology, Beijing, 100190, China 25794. Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Centre for Nanoscience and Technology, Beijing, 100190, China
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Abstract: | An important and difficult issue is simultaneously identifying the detailed locations of various molecules on the cell surface, as this identification requires a synergistic effect between more than one molecule in a living cell. Au nanoparticles (NPs) with different shapes can be readily recognised under low vacuum scanning electron microscopy (lvSEM). Anisotropic Au nanorods (NRs) possess unique surface plasmon resonance (SPR) properties, which can be further utilised for two photon luminescence (TPL) and other optical imaging techniques. In this paper, Au NRs and Au nanooctahedra (Au NOs) are introduced as biomarkers for ICAM-1 and Integrin β1. Combined with the advantages of lvSEM, this multiple-labelling method is a new method for studying the interactions between specific, functional molecules. |
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