Patch and whole-cell voltage clamp of single mammalian visceral and vascular smooth muscle cells |
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Authors: | T. B. Bolton R. J. Lang T. Takewaki C. D. Benham |
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Affiliation: | (1) Department of Pharmacology, St George's Hospital Medical School, SW17 ORE London, (England) |
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Abstract: | Summary Dispersal of the constituent cells of mammalian visceral and vascular smooth muscles has permitted recordings both of membrane currents under whole-cell voltage clamp, and of currents through single ionic channels using the patch-clamp technique. A rectangular depolarizing step applied to a single cell under voltage clamp yielded a net inward current followed by a net outward current in normal physiological solution. In isolated, inside-out patches of cell membrane a calcium- and potential-sensitive K channel (100 pS conductance) and a calcium-insensitive, potential-sensitive K+ channel (50 pS conductance) with slow kinetics have so far been identified and characterized. |
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Keywords: | smooth muscle K channels patch clamp |
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