Redundant and unique roles of coronin proteins in Dictyostelium |
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| Authors: | Maria C. Shina Annette Müller-Taubenberger Can Ünal Michael Schleicher Michael Steinert Ludwig Eichinger Rolf Müller Rosemarie Blau-Wasser Gernot Glöckner Angelika A. Noegel |
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| Affiliation: | 1.Institute for Biochemistry I, Center for Molecular Medicine Cologne (CMMC) and Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), Medical Faculty,University of Cologne,Cologne,Germany;2.Institute for Cell Biology and Center for Integrated Protein Science (CIPSM),Ludwig Maximilians University Munich,Munich,Germany;3.Institute for Microbiology,Technical University Braunschweig,Brunswick,Germany;4.Leibniz Institute for Freshwater Ecology and Inland Fisheries,Berlin,Germany;5.Institute for Biochemistry I, Medical Faculty,University of Cologne,Cologne,Germany;6.Medical Microbiology, Department of Laboratory Medicine,Malm?, University Hospital, Lund University,Malm?,Sweden |
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| Abstract: | Dictyostelium discoideum harbors a short (CRN12) and a long coronin (CRN7) composed of one and two beta-propellers, respectively. They are primarily present in the cell cortex and cells lacking CRN12 (corA −) or CRN7 (corB −) have defects in actin driven processes. We compared the characteristics of a mutant cell line (corA − /corB −) lacking CRN12 and CRN7 with the single mutants focusing on cytokinesis, phagocytosis, chemotaxis and development. Cytokinesis, uptake of small particles, and developmental defects were not enhanced in the corA − /corB − strain as compared to the single mutants, whereas motility and phagocytosis of yeast particles were more severely impaired. It appears that although both proteins affect the same processes they do not act in a redundant manner. Rather, they often act antagonistically, which is in accordance with their proposed roles in the actin cytoskeleton where CRN12 acts in actin disassembly whereas CRN7 stabilizes actin filaments and protects them from disassembly. |
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