Chemical characterization of human urine albumin in proteinuria

Summary From the urine of a patient with proteinuria, the albumin protein component was isolated and compared with human serum albumin. By comparing the amino acid composition of the original proteins and their large cyanogen bromide fragments, peptide maps and N-terminal sequences of 33 amino acid residues, the identity of both proteins was shown.We wish to express our thanks to Mr L. Slepika and Dr A. Koent for the protein fractionation. We are indebted to Mr K. Grüner for the Edman degradation experiments, to Mrs E. Drková for amino acid analyses and Mrs A. Kulhánková for technical assistance.