Purification of a nuclease from human serum |
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| Authors: | E. J. Zöllner G. Seibert H. Slor R. K. Zahn |
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| Affiliation: | (1) Physiologisch-Chemisches Institut der Johannes-Gutenberg-Universität, D-6500 Mainz, (Federal Republic of Germany);(2) Department of Human Genetics, Tel Aviv University, Tel Aviv, (Israel);(3) Present address: Hoechst AG, D-6230 Frankfurt/Main 80, (FRG) |
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| Abstract: | Summary The purification procedure for a nuclease from human serum is described. It includes ammonium sulfate precipitation, chromatography on DEAE-Sephadex and on Sephacryl-S 200, and preparative electrophoresis. The enzyme, purified about 2000-fold, is homogeneous in a sodium dodecyl sulfate electrophoretic system, where it has a mol. wt of 78,000. The pH optimum lies around pH 6.5; it is a sugar-nonspecific endonuclease.Acknowledgment. This work has been supported by a research grant from Stiftung Volkswagenwerk through the Akademie der Wissenschaften und der Literatur, Mainz. We thank Mrs R. Nehrbass and Mrs C. Wolpert for technical assistance. |
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