| 绿原酸对脂多糖致炎症小鼠体内体外COX-2的影响 |
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| 作者单位： | ;1.黄河科技学院医学院;2.南京师范大学生命科学学院 |
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| 摘 要： | 探讨绿原酸对脂多糖致炎症小鼠体内体外COX-2蛋白表达的影响.脂多糖滴鼻造模建立小鼠急性肺损伤模型,脂多糖诱导小鼠巨噬细胞RAW264.7建立细胞炎症反应模型,应用Weston blot法,检测小鼠肺组织内以及RAW264.7巨噬细胞中COX-2的蛋白表达.绿原酸在小鼠体内体外均能有效降低脂多糖诱导的COX-2的蛋白表达,并能促进COX-2蛋白的降解.降低脂多糖诱导的COX-2的蛋白水平是绿原酸抗炎的机制之一.
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| 关 键 词： | 绿原酸 急性肺损伤 COX-2 |
Effect of Chlorogenic Acid on LPS-induced COX-2 in Vivo and Vitro |
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| Affiliation: | ,School of Medicine,Huanghe College of Science and Technology,College of Life Science,Nanjing Normal University |
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| Abstract: | The object of this study was to explore the effect of chlorogenic acid on LPS-induced COX-2 in vivo and vitro.The mice induced by LPS was used as an acute lung injury model.The RAW264.7 cells induced by LPS was used as an cell inflammatory model.The expression of COX-2in lung tissue as well as in RAW264.7 cells was detected by Western blot.The results showed that chlorogenic acid could inhibit the expression of COX-2 protein in vivo and vitro effectively and promote the protein degradation of COX-2 protein in RAW264.7 cells.Reducing the protein level of LPS-induced COX-2 is one of the mechanisms for the anti-inflammatory action of chlorogenic acid. |
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| Keywords: | chlorogenic acid acute lung injury COX-2 |
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