拟南芥类结瘤素基因At1g01070的生物信息学分析

类结瘤素基因在非结瘤植物生长发育中承担着重要的功能,但仅少数基因的功能被鉴定.本研究对拟南芥类结瘤素MtN21(Medicago truncatula NODULIN 21)家族成员At1g01070进行了生物信息学分析及亚细胞定位验证.结果表明,At1g01070有两种可变剪切体,编码两种蛋白At1g01070.1和At1g01070.2,前者比后者在N端多47个氨基酸,它们的分子量分别为40KD和35KD,等电点分别为9.2和8.9;均含有2个MtN21蛋白的特征结构域EamA(药物/代谢物转运结构域),At1g01070.1比At1g01070.2多一个PLN00411结构域,且其靠近N端的EamA结构域较后者长;三级结构均由7个α-螺旋和一些不规则卷曲组成,分别含有10个和8个疏水跨膜区,均含13个磷酸化修饰位点,亚细胞定位预测主要定位在质膜;进化上,与琴叶拟南芥(Arabidopsis lyrata)亲缘关系最近;亚细胞定位结果显示At1g01070.1定位在质膜,与预测结果一致.推测At1g01070在植物体内可能参与物质运输.

Bioinformatic Analysis of Nodulin-like Gene At1g01070 in Arabidopsis thaliana

Recent studies highlight the importance of nodulin-like genes during development of plants that are unable to nodulate.However,only few gene functions were identified.In this study,At1g01070,a member of MtN21 gene family in Arabidopsis thaliana,was analyzed with bioinformatics methods and the subcellular localization of it was determined.The results showed that At1g01070 undergoes alternative splicing to produce two splice variants encoding At1g01070.1 and At1g01070.2respectively.The molecular weights of At1g01070.1and At1g01070.2are 40 KD and 35 KD respectively.The isoelectric points of them are 9.2and 8.9respectively.The two proteins both contain two drug/metabolite domains named EamA,a characteristic domain of MtN21 protein family.At1g01070.1has a specific PLN00411 domain and its EamA domain at N terminal is longer than that of At1g01070.2.The two proteins have similar tertiary structure containing sevenα-helicals and some random coils.They exhibit strong hydrophobicity with ten and eightα-helical transmembrane-spanning domains respectively.There were thirteen phosphorylation sites in At1g01070 protein.The two proteins are predicted to localize to the plasma membrane most likely.To confirm this,At1g01070.1::GFP fusion protein was transiently expressed in tobacco.The observed results were in agreement with the prediction.Phylogenetic analysis showed that At1g01070 had the highest evolutionary relationship with predicted protein in Arabidopsis lyrata.The results suggested that At1g01070 may be involved in the transport of solutes in plants.