| ITN4-C相互作用蛋白的筛选与鉴定 |
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| 引用本文： | 唐小军 陈益存 张锡然. ITN4-C相互作用蛋白的筛选与鉴定[J]. 南京师大学报(自然科学版), 2007, 30(1): 74-77 |
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| 作者姓名： | 唐小军 陈益存 张锡然 |
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| 作者单位： | 南京师范大学生命科学学院,江苏省分子医学生物技术重点实验室,江苏南京210097 |
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| 摘 要： | 我们的前期工作发现过表达RTN4-C基因能够抑制肝癌细胞株SMMC7721的生长并且促进其发生凋亡．为了进一步了解RTN4-C促进凋亡的机制，本文采用酵母双杂交的方法从小鼠7d胚胎文库中筛选其相互作用的蛋白．最终筛选到其同源家族成员RTN3．进一步采用间接免疫荧光和及构建的荧光载体表明，RTN4-C和RTN3蛋白在HEK293和Hela细胞中发生共分布．本研究为进一步阐明RTN4-C抑制细胞生长和促进细胞凋亡的机制提供了新的依据．
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| 关 键 词： | RTN4-C RTN3 酵母双杂交 免疫荧光 共定位 |
| 文章编号： | 1001-4616（2007）01-0074-04 |
| 收稿时间： | 2006-06-06 |
| 修稿时间： | 2006-09-28 |
Screening and Identification of the RTN4 -C Interactive Proteins |
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| Tang Xiaojun, Chen Yicun, Zhang Xiran. Screening and Identification of the RTN4 -C Interactive Proteins[J]. Journal of Nanjing Normal University(Natural Science Edition), 2007, 30(1): 74-77 |
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| Authors: | Tang Xiaojun Chen Yicun Zhang Xiran |
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| Affiliation: | School of Life Science, Nanjing Normal University, The Key Laboratory of Molecular and Medical Biotechnology of Jiangsu Province, Nanjing 210097, China |
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| Abstract: | Our previous study showed that overexpression of the RTN4 - C might inhibit SMMC7721 cell growth and promote cell apoptosis. To investigate the mechanism of RTN4 - C inhibition of cell growth and pro-apoptosis, the yeast two-hybrid method was adopted. By screening the mouse 7 - day Embryo MATCHMAKER cDNA Library, we obtained the interacted protein RTN3, which has been reported to pro-apoptosis. Our further study indicated that RTN4 - C and RTN3 colocalized in HEK293 and Hela cells using immunofluorescence and fluorescence vectors. The present study could be useful to clarify the mechanism of RTN4 - C inhibition of cell growth and pro-apoptosis. |
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| Keywords: | RTN4- C RTN3 yeast two-hybrid immunofluorescence co-locolization |
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