| 脱氮假单胞菌发酵生产维生素B12高产菌株的选育 |
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| 引用本文： | 祁桂玲,宋毅芳. 脱氮假单胞菌发酵生产维生素B12高产菌株的选育[J]. 河北省科学院学报, 2006, 23(3): 16-18 |
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| 作者姓名： | 祁桂玲 宋毅芳 |
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| 作者单位： | 华北制药股份有限公司,河北,石家庄,050015;华北制药股份有限公司,河北,石家庄,050015 |
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| 摘 要： | 以维生素B12生产菌株脱氮假单胞菌05-04为出发菌株,采用紫外线和5-澳尿嘧啶复合诱变处理,选育到一株抗5-澳尿嘧啶突变株5-BU-3-5,该菌株经摇瓶发酵,发酵单位较出发菌株提高了18.0%.该菌株特性优良,经57m3发酵罐试验,平均发酵单位提高8.9%,是一株性状较稳定可深入开发研究的优良菌株.
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| 关 键 词： | 维生素B12 脱氮假单胞菌 菌种选育 突变株 |
| 文章编号： | 1001-9383(2006)03-0016-03 |
| 修稿时间： | 2006-06-10 |
Selection of fermention produce vitamin B12 strain from denitrogenation pseudomonas |
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| QI Gui-ling,SONG Yi-fang. Selection of fermention produce vitamin B12 strain from denitrogenation pseudomonas[J]. Journal of The Hebei Academy of Sciences, 2006, 23(3): 16-18 |
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| Authors: | QI Gui-ling SONG Yi-fang |
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| Abstract: | As an original strain,denitrogenation pseudomonas 05-04 was mutated by UV and 5-BU.One strain 5-BU-3-5 was obtained.During the shake flask experiment,the ferment unit of strain 5-BU-3-5 was found increasing 18% than original strain ferment unit.During 57m~3 ferment experiment,the average ferment unit was increased 8.9%.It is a equiform strain,which can research more. |
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| Keywords: | Vitamin B12 Pseudomonas denitrificans The germ grows the seed selection Chop round the stub |
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