| Analysis of Male Sterility-Related Proteins of Young Panicle in Rice （Oryza sativa L. ） by Two-Dimensional Electrophoresis |
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| 摘 要： |
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| 关 键 词： | 杂交水稻 蛋白质 电泳现象 聚丙烯酰胺 |
| 收稿时间： | 7 July 2004 |
Analysis of male sterility-related proteins of young panicle in rice (Oryza sativa L.) by two-dimensional electrophoresis |
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| Authors: | Hu Yao-jun Wei Lei Liu Shu-nan Yu Jin-hong Ding Yi |
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| Institution: | (1) Key Laboratory of MOE for Plant Developmental Biology/College of Life Sciences, Wuhan University, 430072 Wuhan, Hubei, China;(2) College of Life Sciences, Hunan University of Science and Technology, 411201 Xiangtan, Hunan, China;(3) College of Life Sciences, Huazhong Normal University, 430079 Wuhan, Hubei, China |
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| Abstract: | For searching out male sterility-related proteins (polypeptides) in rice (Oryza sativa L.), we examined the difference of panicle protein (polypeptides) between hybrid rice (Wujin2A/R168, Wujin5A/R988) and their
parents (male-sterile line Wujin2A, Wujin5A, and restorer line R168, R988) at the formation stage of pollen mother cell by
two-dimensional electrophoresis (2-DE). The results revealed that the 2-DE polypeptide maps were similar among these experimental
materials. A small group of polypeptides were disappeared in 2-DE polypeptide maps of male-sterile line (Wujin2A, Wujin5A)
by comparing to restorer line (R168, R988) and the first filial (F1) generation (Wujin2A/R168, Wujin5A/R988). The isoelectric points of these polypeptides were pI 5.8–6.5 molecular, weight
42.7×103−66.2×103.
Foundation item: Supported by the National Natural Science Foundation of China (30340079) and the Science and Technology Key Research Foundation
of the Education Ministry of China (03109)
Biography: HU Yao-jun (1960-), male Ph. D candidate, research direction: molecular cytogenetics. |
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| Keywords: | hybrid rice male-sterile protein two-dimensional polyacrylamide gel electrophoresis |
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