| Multiple transgenes Populus xeuramericana 'Guariento' plants obtained by biolistic bombardment |
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| 作者单位： | Wang JianGe1，2，Su XiaoHua1，Ji LiLi1，Zhang BingYu1，Hu ZanMin3，Huang RongFeng4 & Tian YingChuan5 1 Research Institute of Forestry，Chinese Academy of Forestry，Beijing 100091，China; 2 College of Landscape and art，Jiangxi Agricultural University，Nanchang 330045，China; 3 Institute of Genetics and developmental biology，Chinese Academy of Sciences，Beijing 100101，China; 4 Biotechnology Research Institute，Chinese Academy of Agricultural Science，Beijing 100081，China; 5 Microbiology Institute，Chinese Academy of Sciences，Beijing 100080，China |
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| 摘 要： | A JERF36 regulation gene, a selection marker gene (NPT-II), and the foreign genes levansucrase (SacB), Vitreoscilla hemoglobin (vgb), and Binary coleopterus insect resistance (BtCry3A OC-I) were co-transferred into Populus xeuramericana 'Guariento' using biolistic bombardment; 25 kanamycin resistant plants were obtained. The results of PCR and Southern hybridization showed that the foreign genes had been integrated into the genome of P. xeuramericana 'Guariento' and 5 genes were all transferred into 7 poplar plants. The results of a BtCry3A ELISA experiment indicated that the BtCry3A gene was expressed in the 7 transgenic poplar plants, and these plants grew well on coastal saline land.
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Multiple transgenes Populus xeuramericana 'Guariento' plants obtained by biolistic bombardment |
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| Authors: | Wang JianGe Su XiaoHua Ji LiLi Zhang BingYu Hu ZanMin Huang RongFeng Tian YingChuan |
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| Abstract: | A JERF36 regulation gene, a selection marker gene (NPT-II), and the foreign genes levansucrase (SacB), Vitreoscilla hemoglobin (vgb), and Binary coleopterus insect resistance (BtCry3A OC-I) were co-transferred into Populus xeuramericana 'Guariento' using biolistic bombardment; 25 kanamycin resistant plants were obtained. The results of PCR and Southern hybridization showed that the foreign genes had been integrated into the genome of P. xeuramericana 'Guariento' and 5 genes were all transferred into 7 poplar plants. The results of a BtCry3A ELISA experiment indicated that the BtCry3A gene was expressed in the 7 transgenic poplar plants, and these plants grew well on coastal saline land. |
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| Keywords: | Populus xeuramericana 'Guariento' biolistic bombardment multi-gene co-transformation transgene |
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