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| Imaging of apoptotic HeLa cells by using scanning near-field optical microscopy | |
| 作者姓名： | SHEN Yumin XU Shif LIU Xiaoling LU Ping HAO Jingshan ZHAI Zhonghe Peter von BLANCKENHAGEN ZHU Xing |
| 作者单位： | State Key Laboratory for Mesoscopic Physics and Department of Physics, Peking University, Beijing 100871, China,State Key Laboratory for Mesoscopic Physics and Department of Physics, Peking University, Beijing 100871, China,College of Life Sciences, Peking University, Beijing 100871, China,College of Life Sciences, Peking University, Beijing 100871, China,State Key Laboratory for Mesoscopic Physics and Department of Physics, Peking University, Beijing 100871, China,College of Life Sciences, Peking University, Beijing 100871, China,Institute of Nanotechnology, Research Center Karlsruhe, Karlsruhe D-76021, Germany,State Key Laboratory for Mesoscopic Physics and Department of Physics, Peking University, Beijing 100871, China |
| 基金项目： | Supported by the National Natural Science Foundation of China (Grant Nos. 19890380， 10074002， 30070388) and the Research Fund for the Doctoral Program of Higher Education, Ministry of Education, China |
| 摘 要： | By using scanning near-field optical microscopy (SNOM), HeLa cells in apoptosis process are imaged with a higher optical resolution beyond the diffraction limit. Since SNOM provides both topographic and transmitted light intensity information of a cell, it can correlate the structural characteristics and optical properties with the spatial position of the apoptotic cells. Wavelength imaging by using near-field spectroscopy shows that there is a great difference in light propagation and absorption in the cell. This unique technique can be applied to the super high resolution imaging of different components in the cell. The observations by near-field optical imaging and near-field spectroscopy indicate an inhomogeneous aggregation of the inner structure in the apoptotic HeLa cells and the change of transmission intensity of light with the apoptosis status. |
| 关 键 词： | scanning near-field optical microscopy HeLa cell apoptosis spectrum imaging tapping mode |
Imaging of apoptotic HeLa cells by using scanning near-field optical microscopy |
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| SHEN Yumin,XU Shif,LIU Xiaoling,LU Ping,HAO Jingshan,ZHAI Zhonghe,Peter von BLANCKENHAGEN,ZHU Xing.Imaging of apoptotic HeLa cells by using scanning near-field optical microscopy[J].Progress in Natural Science,2002,12(8):577-582. | |
| Authors: | Shen Yumin Xu Shifa LIU Xiaoling LU Ping HAO Jingshan ZHAI Zhonghe Peter von Blanckenhagen Zhu Xing |
| Institution: | 1. State Key Laboratory for Mesoscopic Physics and Department of Physics, Peking University, Beijing 100871, China 2. College of Life Sciences, Peking University, Beijing 100871, China 3. Institute of Nanotechnology, Research Center Karlsruhe, Karlsruhe D-76021, Germany |
| Abstract: | By using scanning near-field optical microscopy (SNOM), HeLa cells in apoptosis process are imaged with a higher optical resolution beyond the diffraction limit. Since SNOM provides both topographic and transmitted light intensity information of a cell, it can correlate the structural characteristics and optical properties with the spatial position of the apoptotic cells. Wavelength imaging by using near-field spectroscopy shows that there is a great difference in light propagation and absorption in the cell. This unique technique can be applied to the super high resolution imaging of different components in the cell. The observations by near-field optical imaging and near-field spectroscopy indicate an inhomogeneous aggregation of the inner structure in the apoptotic HeLa cells and the change of transmission intensity of light with the apoptosis status. |
| Keywords: | scanning near-field optical microscopy HeLa cell apoptosis spectrum imaging tapping mode |
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