| SAS软件对曲霉M-18产内切葡聚糖酶的发酵条件优化 |
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| 引用本文： | 罗永婷, 李焱, 孙凌燕, 李新国, 陈军.SAS软件对曲霉M-18产内切葡聚糖酶的发酵条件优化[J].上海师范大学学报(自然科学版),2009,38(3):297-302. |
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| 作者姓名： | 罗永婷 李焱 孙凌燕 李新国 陈军 |
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| 作者单位： | 上海师范大学,生命与环境科学学院,上海,200234 |
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| 摘 要： | 应用单因素优化和响应面分析实验对一株具有产高Cx/FPA比值纤维素酶的曲霉M-18菌株进行发酵条件优化试验,确定了合适的碳源、氮源及发酵控制参数,得到回归方程反映出该模型具有良好的拟合性,可以用于产内切葡聚糖酶发酵优化的理论预测.进一步分析得到的优化发酵条件为:在基础培养基中添加CMC-Na 0.3%,酵母粉0.5%;产酶发酵温度30℃,装液量67mL/250mL摇瓶、转速175 r/m和接种量1.2 cm2菌苔/50mL.优化前后的实验验证结果相比:优化后的摇瓶发酵Cx酶活力达129.62 u/mL,比原来的98.45 u/mL高出31.67%.
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| 关 键 词： | 纤维素酶 Cx酶/TPA 响应面分析方法 发酵条件优化 |
Condition optimization for Cx cellulase of Aspergillus M-18 by SAS software |
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| LUO Yong-ting,LI Yan,SUN Ling-yan,LI Xin-guo,CHEN Jun.Condition optimization for Cx cellulase of Aspergillus M-18 by SAS software[J].Journal of Shanghai Normal University(Natural Sciences),2009,38(3):297-302. |
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| Authors: | LUO Yong-ting LI Yan SUN Ling-yan LI Xin-guo CHEN Jun |
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| Institution: | College of Life and Environment Sciences;Shanghai Normal University;Shanghai 200234;China |
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| Abstract: | The fermentation conditions of a high-yield Cx cellulose of Aspergillus M-18 were optimized by using single factor optimization experiment,the Plackett-Burman design and the response surface analysis by SAS software.Some data including the concentration of carbon source,nitrogen source and the value of fermentation parameter were determined.Then a regression equation was obtained.This regression equation accorded with the result of experiment.So it could be used for forecasting optimization results of exper... |
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| Keywords: | Cellulase CMCase/FPA response surface analysis fermentation condition optimization |
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