Nitric oxide damages neuronal mitochondria and induces apoptosis in neurons |
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Authors: | Taotao Wei Chang Chen Jingwu Hou Baolu Zhao Wenjuan Xin |
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Institution: | (1) Institute of Biophysics, Chinese Academy of Sciences, 100101 Beijing, China |
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Abstract: | The cytotoxic effect of nitric oxide on primarily cultured rat cerebellar granule cells was studied, and the mechanisms were
discussed. The results showed that nitric oxide donor S-nitroso-N-acetyl-penicillamine (SNAP; 500 μmol/L) could induce apoptosis
in immature cultures of cerebellar granule cells. Flow cytometry and HPLC analyses revealed that after treatment with SNAP,
the mitochondrial transmembrane potential and the cellular ATP content decreased significantly. Nitric oxide scavenger hemoglobin
could effectively prevent the neuronal mitochondria from dysfunction and attenuate apoptosis. The results suggested that nitric
oxide activated the apoptotic program by inhibiting the activity of mitochondrial respiratory chain and thus decreasing the
cellular ATP content. |
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Keywords: | cerebellar granule cells apoptosis nitric oxide mitochondria |
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