| 丁酸梭菌产1,3-丙二醇的培养基优化 |
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| 引用本文： | 熊玮,朱春杰,方柏山. 丁酸梭菌产1,3-丙二醇的培养基优化[J]. 厦门大学学报(自然科学版), 2012, 51(5): 893-897 |
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| 作者姓名： | 熊玮 朱春杰 方柏山 |
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| 作者单位： | 1. 厦门大学化学化工学院,福建厦门,361005
2. 厦门大学化学化工学院,福建厦门361005;厦门市合成生物技术重点实验室,福建厦门361005 |
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| 基金项目： | 国家自然科学基金项目(21076172) |
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| 摘 要： | 为提高丁酸梭菌(Clostridium butyricum)VPI 1718产1,3-丙二醇的能力,采用均匀设计试验和人工神经网络结合遗传算法对培养基进行优化,得到最优配方(g/L):废甘油70,酵母粉2.0,KH2PO4 0.6,K2HPO4 1.2,(NH4)2SO42.6,MgSO4 0.2;使用该培养基在5 L发酵罐中分批培养14 h丁酸梭菌后,1,3-丙二醇质量浓度为33.0g/L,转化率为55.3%,生产强度为2.36 g/(L·h),较优化前分别提高了32.5%,7.17%和33.3%.
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| 关 键 词： | 丁酸梭菌 1,3-丙二醇 均匀设计 人工神经网络 遗传算法 |
Medium Optimization for 1,3-Propanediol Production by Clostridium butyricum |
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| XIONG Wei , ZHU Chun-jie , FANG Bai-shan. Medium Optimization for 1,3-Propanediol Production by Clostridium butyricum[J]. Journal of Xiamen University(Natural Science), 2012, 51(5): 893-897 |
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| Authors: | XIONG Wei ZHU Chun-jie FANG Bai-shan |
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| Affiliation: | 1,2(1.College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China;2.The Key Lab for Synthetic Biotechnology of Xiamen,Xiamen 361005 ,China) |
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| Abstract: | In the present work, uniform design and artificial neural network linked genetic algorithm were applied to optimize 1,3-propanediol fermentation medium to enhance 1,3-propanediol production by Clostridium butyricum VPI 1718. The compositions of the optimized fermentation medium were as follows (g/L):raw glycerol 70, yeast extract 2.0, K2H PO4 1.2,KH2PO4 0.6,(NH4)2SO4 2.6,Mg SO4 0.2. When the C. butyricum was cultivated in the optimized medium for 14 h on a 5 L fermentor,the 1,3-propanediol concentration, yield and productivity were 33.0 g/L,55.3% and 2.36 g/(L·h),which were 32.5 % , 7.17 % and 35.6 % highter than that in the initial medium, respectively. |
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| Keywords: | Clostridium butyricum 1,3-propanediol uniform design artificial neural network genetic algorithm |
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