A new approach to analysis of human sweating |
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| Authors: | M Shimazu T Matsumoto M Kosaka N Ohwatari K Tsuchiya Y Ueyama K Urano Y Kataki M Saito |
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| Institution: | (1) Department of Environmental Physiology, Institute of Tropical Medicine, Nagasaki University, 852 Nagasaki, (Japan);(2) Kanagawa Academy of Science and Technology, 213 Kanagawa, (Japan);(3) Central Insititute for Experimental Animals, 213 Kanagawa, (Japan);(4) Department of pathology, School of Medicine, Tokai University, 259-11 Kanagawa, (Japan);(5) Present address: Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, 852 Nagasaki, (Japan) |
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| Abstract: | In human skin transplanted to the back of 3 strains of immuno-deficient mice the functin of the eccrine sweat glands of the human transplant was tested by topical intradermal application of pilocarine, adrenaline and atropine+pilocarpine. Sweat responses were observed in pre-selected fields of observation by means of video macroscope. The iodine strarch reaction served as an indicator for the appearance of seat sport and permitted the evaluation of areas wetted by sweat in the field of observation. Among 9 animals tested, the hybrids between the CB-17-scid mouse and the BALB/cA-nu mouse (BALB/cA-nu,scid) seemed to exhibit the most consistent seweating response to local pharmacological stimulation. According to histological examination, eccrine sweat glands were preserved in human skin trasplanted into the back skin of the BALB/cA-nu,scid mouse strain. the heterologous, human skin graft provides a novel model permitting, independent of the normal sweat gland innervation, the analysis of moecular receptors of sweat gland cells by which the actions of natural transmitters and pharmacological agents are transduced. |
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| Keywords: | Human skin heterologous transplant immuno-deficient mouse eccrine seating pilocarpine adrenaline atropine |
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