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| 采用染料亲和层析的方法纯化卵泡刺激素 | |
| 引用本文： | 刘志勇,王启要,龚雁,张万山.采用染料亲和层析的方法纯化卵泡刺激素[J].江西科学,2004,22(1):11-14. |
| 作者姓名： | 刘志勇 王启要 龚雁 张万山 |
| 作者单位： | 1. 江西省劳动卫生研究所,江西,南昌,330006 2. 南昌市万华生化制品有限公司,江西,南昌,330029 3. 江西省生物科技学院,江西,南昌,330200 |
| 摘 要： | 采用染料亲和层析、QAE离子交换层析等纯化步骤，从绝经期妇女尿促性腺激素(HMG)粗品中分离纯化获得高纯度卵泡刺激素(FSH)产品，其中FSH的生物活性达到268.1IU／mg，卵泡刺激素与黄体生长素(LH)的活性比值达到259，产品超过欧洲药典对高纯度卵泡刺激素的要求。此工艺FSH的活性回收率高达65％，而且分离纯化步骤少，简单易行，适合于大规模生产。 |
| 关 键 词： | 染料亲和层析 卵泡刺激素 QAE离子交换层析 人尿促性腺激素 染料亲和层析 |
| 文章编号： | 1001-3679(2004)01-0011-04 |
| 修稿时间： | 2003年8月1日 |
Purification of Follicle Stimulating Hormone by dye Affinity Chromatography |
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| LIU Zhi_yong,WANG Qi_yao,GONG Yan,ZHANG Wan_shan.Purification of Follicle Stimulating Hormone by dye Affinity Chromatography[J].Jiangxi Science,2004,22(1):11-14. | |
| Authors: | LIU Zhi_yong WANG Qi_yao GONG Yan ZHANG Wan_shan |
| Institution: | LIU Zhi_yong~1,WANG Qi_yao~2,GONG Yan~3,ZHANG Wan_shan~2 |
| Abstract: | High purity follicle stimulating hormone(FSH)was produced from human menopausal gonadotropin(HMG)crude extracts by utilizing a new purification design in which dye affinity chromatography(DAC)along with QAE ion_exchange chromatography was used to greatly enhance the purity and bioactivity. The bioactivity of the FSH product reached 268.1IU/mg and the ratio between FSH and leuteinizng hormone(LH)amounted to 259, which allowed for the FSH product to comply with the UP standards set for high purity FSH products. With a total recovery rate as high as 65% for the FSH bioactivity,this purification process will be applicable to large scale manufacturing of FSH. |
| Keywords: | Follicle stimulating hormone Dye affinity chromatography QAE ion_exchange chromatography Human menopausal gonadotropin |
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