胰岛素抵抗3T3-L1脂肪细胞中脂联素mRNA表达的研究 |
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引用本文: | 郭晓农. 胰岛素抵抗3T3-L1脂肪细胞中脂联素mRNA表达的研究[J]. 西北民族学院学报, 2010, 31(1) |
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作者姓名: | 郭晓农 |
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作者单位: | 西北民族大学,生命科学与工程学院,甘肃,兰州,730030 |
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基金项目: | 西北民族大学科研项目 |
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摘 要: | 目的:通过本实验研究脂联素mRNA在胰岛素抵抗3T3-L1脂肪细胞中的表达.方法:培养3T3-L1前脂肪细胞,诱导分化为脂肪细胞,采用地塞米松诱导建立3T3-LI脂肪细胞胰岛素抵抗模型,运用RT-PCR技术检测脂联素mRNA的表达.结果:随着胰岛素抵抗的发生,脂联素mRNA的表达量明显降低,经过罗格列酮干预胰岛素抵抗后,干预组脂联素mRNA表达量较空白组升高了约103.5%.结论:脂联素mRNA表达量与胰岛素抵抗的发生关系密切,其有望作为治疗胰岛素抵抗的靶基因.
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关 键 词: | 脂联素 胰岛素抵抗 3T3-LI脂肪细胞 mRNA |
Study on Expression of Adiponectin mRNA in Insulin Resistance 3T3-L1 Adipocytes |
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Abstract: | Objective:To investigate the expression of adiponectin mRNA in insulin-resistant 3T3-L1 adipocytes through the empirical study.Methods:3T3-L1 preadipocytes were cultured and induced to differentiation.To establish insulin resistant 3T3-L1 adipocytes cell model induced by dexamethasone and to detect the expression of adiponectin mRNA deploy RT-PCR.Results:Following the proceed of insulin-resistant,the expression of adiponectin mRNA was significantly decreased.The expression of adiponectin mRNA in interventional group step up about 103.5% compared with the blank group after rosiglitazone intervention.Conclusions:The relationship was close between the expression of adiponectin mRNA and the proceed of insulin-resistant,adiponectin could conduct and actions as target gene on treat insulin-resistant. |
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Keywords: | mRNA |
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