| 利用低强度脉冲电磁场对家猪精子电穿孔的研究 |
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| 引用本文： | 王小行,彭勇,王子淑,王保义,张弘,刘长军.利用低强度脉冲电磁场对家猪精子电穿孔的研究[J].四川大学学报(自然科学版),2002,39(5):934-937. |
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| 作者姓名： | 王小行 彭勇 王子淑 王保义 张弘 刘长军 |
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| 作者单位： | 1. 四川大学生命科学学院,成都,610064
2. 四川大学无线电系,成都,610064 |
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| 基金项目： | 国家自然科学基金 (6 0 0 710 2 4) |
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| 摘 要： | 用低强度脉冲电磁场对家猪精子细胞进行电击处理，发现台盼蓝染料能够进入细胞，而且通过扫描电镜可以观察到细胞膜表面有明显的孔洞。这证实了低强度瞬态电磁场能够引起猪精子细胞电穿孔和促使外源物质进入细胞内。
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| 关 键 词： | 家猪 低强度脉冲电磁场 精子 电穿孔 转基因动物 DNA 电击法 |
| 文章编号： | 0490-6756(2002)05-0934-04 |
Study on the Effects of Electroporation of Domestic Pig Sperm Cell Induced by Weak Electric-Magnetic Pulse Field |
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| WANG Xiao-xing ,PENG Yong ,WANG Zi-shu ,WANG Bao-yi ,ZHANG Hong ,LIU Chang-jun.Study on the Effects of Electroporation of Domestic Pig Sperm Cell Induced by Weak Electric-Magnetic Pulse Field[J].Journal of Sichuan University (Natural Science Edition),2002,39(5):934-937. |
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| Authors: | WANG Xiao-xing PENG Yong WANG Zi-shu WANG Bao-yi ZHANG Hong LIU Chang-jun |
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| Institution: | WANG Xiao-xing 1,PENG Yong 1,WANG Zi-shu 1,WANG Bao-yi 2,ZHANG Hong 2,LIU Chang-jun 2 |
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| Abstract: | The sperms of domestic pig were freated with weak electric-magneti pulse field. The authors observe that Try-Pan blue can enter the sperm cells and there are obvious holes in the cell surface. It confirms that weak electric-magnetic pulse field can cause electroporation of domestic pig sperms and make the little exogenous molecules enter the sperm cells. |
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| Keywords: | weak electric-magnetic pulse field sperm electroporation |
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