Photoautotrophic Growth and Net Photosynthetic Rate of Sweet Potato Plantlets In Vitro as Affected by the Number of Air Exchanges of the Vessel and Type of Supporting Material |
| |
| Authors: | XIAO Yulan Kozai Toyoki |
| |
| Institution: | 1. Faculty of Horticulture, Chiba University, Matsudo, Chiba 271-8510, Japan;2. Yangtze Delta Region Institute of Tsinghua University, Zhejiang, Jiaxing 314100, China;1. Department of Mechanical Engineering, Amirkabir University of Technology, Tehran, Iran;2. Department of Mechanical Engineering, Shahid Bahonar University of Kerman, Kerman, Iran;3. Department of Engineering Design and Materials, NTNU, Trondheim, Norway;1. Institute of Theoretical Physics, Xinyang Normal University, Xinyang, Henan 464000, China;2. Institute of Particle Physics and Key Laboratory of Quark and Lepton Physics (MOE), Central China Normal University, Wuhan, 430079, China;3. Department of Physics and Astronomy, Wayne State University, Detroit, MI 48201, USA;1. Department of Physics and Astronomy, University of California, Irvine, CA 92697-4575, USA;2. Instituto de Física, Universidad Nacional Autónoma de México, POB 20-364, Cd.Mx. 01000, Mexico;3. Physik Department T75, Technische Universität München, James-Franck-Straße 1, 85748, Garching, Germany;1. Department of Obstetrics and Gynecology, MacKay Memorial Hospital, Taipei, Taiwan;2. Department of Medical Research, MacKay Memorial Hospital, Taipei, Taiwan;3. Department of Biotechnology, Asia University, Taichung, Taiwan;4. School of Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung, Taiwan;5. Institute of Clinical and Community Health Nursing, National Yang-Ming University, Taipei, Taiwan;6. Department of Obstetrics and Gynecology, School of Medicine, National Yang-Ming University, Taipei, Taiwan;7. Genephile Bioscience Laboratory, Ko''s Obstetrics and Gynecology, Taipei, Taiwan;8. Department of Pediatrics, MacKay Memorial Hospital, Taipei, Taiwan;9. Department of Medicine, MacKay Medical College, New Taipei City, Taiwan;10. Department of Early Childhood Care, National Taipei University of Nursing and Health Sciences, Taipei, Taiwan;11. Gene Biodesign Co. Ltd, Taipei, Taiwan;12. Department of Bioengineering, Tatung University, Taipei, Taiwan |
| |
| Abstract: | To produce high-quality sweet potato plantlets rapidly at low production costs, single nodal leafy cuttings of sweet potato (Ipomoea batatas (L.) Lam.) plantlets were cultured in vitro for 14 days on sugar- and phytohormone-free Murashige and Skoog (MS) liquid medium, at a photosynthetic photon flux (PPF) of 200 μmol · m−2 · s−1 and a CO2 concentration (v/v) of 1.8×10−3. A factorial experiment was conducted with two levels of the number of air exchanges of the vessel (NAE), 8.7-12.2 h−1 and ≥12.2 h−1, and two types of supporting material, vermiculite and Florialite (a porous material). The control treatment consisted of a photomixotrophic culture using a sugar- and NAA-containing agar MS medium with an NAE of 2.4 h−1. PPF and CO2 concentrations were the same as the photoautotrophic treatments. In comparison with the control treatment, the photoautotrophic treatments with NAE of 8.7–12.2 h−1 (MF treatment) and ≥12.2 h−1 (HF treatment) were, respectively, 2.2 and 2.8 times in dry weight, and 3.7 and 4.2 times in net photosynthetic rate, when Florialite was used. The survival percentages of the plantlets in the field were, respectively, 86% and 97% in the MF and HF treatments, and 35% and 46% higher than that in the control treatment. The plantlets cultured with Florialite showed greater growth, compared with those cultured with vermiculite. Photoautotrophic micropropagation system with high NAE and the use of porous supporting material can produce high-quality plantlets and make it possible to reduce production costs. |
| |
| Keywords: | micropropagation photomixotrophic photoautotrophic CO2 enrichment relative humidity sur-vival percentage |
| 本文献已被 CNKI 维普 万方数据 ScienceDirect 等数据库收录！ |
|
