血红蛋白在多孔ZnO 纳米球修饰电极上的直接电化学与电催化

以1-丁基吡啶六氟磷酸盐作为粘合剂制备的离子液体修饰碳糊电极为基底电极,将多孔ZnO纳米球和血红蛋白(Hb)用壳聚糖固定在电极表面制备出电化学酶传感器.紫外可见光谱和傅里叶变换红外光谱实验结果表明Hb在复合膜内保持其基本结构.通过修饰电极在PH 6.0的PBS缓冲溶液中的循环伏安,得到了的一对良好的氧化还原峰,可以证明Hb分子结构中的电活性中心与电极之间发生了直接电子转移,对Hb的电化学行为进行研究,求解了相关的电化学参数,该Hb修饰电极对三氯乙酸表现出良好的电催化活性.

Direct Electrochemistry of Hemoglobin on Mesoporous ZnONanosphere Modified Electrode and Electrocatalysis

Ionic liquid 1-butylpyridinium hexafluorophosphate was used as the modifier to prepare a carbon ionic liquidelectrode and used as the base electrode. Mesoporous ZnO nanosphere and hemoglobin (Hb) were immobilized on the CILEsurface by a chitosan film to get an electrochemical sensor. UV-Vis absorption and FT-IR infrared spectra proved that Hbremained its basic conformation in the composite. Cyclic voltammetric results exhibited a pair of well-defined redox peak inpH 6.0 PBS, which was ascribed to the direct electron transfer of electroactive center of Hb with the working electrode. Electrochemical behaviors of Hb on the modified electrode were studied and the electrochemical parameters were calculated.This Hb modified electrode showed excellent electrocatalytic activity to the reduction of tricholoacetic acid.