| 桑天牛染色体核型及制备材料的选择 |
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| 引用本文： | 刘平,嵇保中,刘曙雯,张凯,于秀坤,林晓.桑天牛染色体核型及制备材料的选择[J].南京林业大学学报(自然科学版),2010,0(6):5-8. |
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| 作者姓名： | 刘平 嵇保中 刘曙雯 张凯 于秀坤 林晓 |
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| 作者单位： | 1. 南京林业大学森林资源与环境学院,江苏,南京,210037
2. 南京中山陵园管理局,江苏,南京,210014 |
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| 基金项目： | 国家林业局科技成果推广项目，南京市建委、中山陵园管理局项目 |
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| 摘 要： | 以桑天牛Apriona germari(Hope)为研究对象，选取桑天牛幼虫神经节，成虫神经节、唾腺、精巢、卵巢、卵胚胎组织进行染色体制备。结果表明制备染色体的最佳试验材料
为2、3日龄胚胎组织。不同日龄胚胎组织的核酸含量随日龄的增加而增大，3、6、9日龄均出现峰值，3日龄时cRNA/cDNA值比6、9日龄时大。胚胎发育期间首次核酸峰值出现时期与
染色体研究最佳材料的选取时间一致，胚胎cRNA/cDNA的值可作为染色体制备材料选择的指标。
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| 关 键 词： | 桑天牛 染色体 核型 胚胎核酸含量 |
The chromosome karyotype and selection of preparation materials in Apriona germari(Hope) |
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| LIU Ping,JI Bao-zhong,LIU Shu-wen,ZHANG Kai,YU Xiu-kun,LIN Xiao.The chromosome karyotype and selection of preparation materials in Apriona germari(Hope)[J].Journal of Nanjing Forestry University(Natural Sciences ),2010,0(6):5-8. |
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| Authors: | LIU Ping JI Bao-zhong LIU Shu-wen ZHANG Kai YU Xiu-kun LIN Xiao |
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| Institution: | LIU Ping1,JI Bao-zhong1,LIU Shu-wen2,ZHANG Kai1,YU Xiu-kun1,LIN Xiao1(1.College of Forest Resources and Environment,Nanjing Forestry University,Nanjing 210037,China,2.The Administration Bureau of Dr.Sun Yat-sen's Mausoleum,Nanjing 210014,China) |
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| Abstract: | Apriona germari(Hope) was chosen as a model to investigate karyotype in this paper.Ganglions of larvae,ganglions,salivary glands,testis and ovaries of adults,tissues of embryo were used as experimental materials.The results showed that the optimal materials were 2,3 days old embryos.The nucleic acid content of embryo was determined.The content was increasing with day age,and peak value appeared at 3,6,9 days old embryo.The ratio of RNA/DNA in 3 days old embryo was higher than that in 6 or 9-days old embryo.... |
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| Keywords: | Apriona germari(Hope) chromosome karyotype content of nucleic acid in embryo |
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