巴西铁的茎组织培养

以巴西铁（Ｄｒａｃａｅｎａ　ｓａｎｄｅｒｉｅｎａ）茎段为接种材料，ＭＳ为基本培养基，吲哚乙酸（ＩＡＡ）、茶乙酸（ＮＡＡ），２，４－二氯苯氧乙酸（２．４－Ｄ）、６－苄氨基嘌呤（６－ＢＡ）、６一糠基氨基嘌呤（６－ＦＡ）、吲哚丁酸（ＩＢＡ）、生根粉（ＡＢＴ１）、玉米素（ＺＴ）为附加成分，进行诱导、分化、生根试验。诱导结果表明：ＩＡＡ不起作用，２，４－Ｄ有较好的促进作用，０．５ｍｇ／Ｌ（２，４－Ｄ）＋２ｍｇ／Ｌ（６－ＢＡ）组合，诱导率高达９０％。分化结果表明：ＮＡＡ、６－ＢＡ效果较好，６－ＦＡ作用不明显，ｌｍｇ／ＬＮＡＡ＋４ｍｓ／Ｌ（６－ＢＡ）＋２ｍｓ／ＬＺＴ组合，分化率最高．达８１．１％。生根结果表明：ＭＳ＋０．５ｍｇ／ＬＮＡＡ＋０．５ｍｇ／Ｌ（６－ＦＡ），ＭＳ＋０．４ｍｇ／ＬＮＡＡ＋２ｍｇ／ＬＩＢＡ，ＭＳ＋０．２ｍｇ／ＬＮＡＡ＋０．１ｍｇ／Ｌ（６－ＦＡ）＋ＡＢＴ１三种培养基均发根，生根率７０％以上，根粗，但有点脆，若培养基中加入活性碳产生的根则细而韧。

Stem Culture in Vitro of Dracaena sanderiena

Stem sections of Dracaena sanderiena as inoculative material, MS as basic culture medium,indoleacetic acid (IAA), naphthyl acetic acid (NAA), 2, 4- dichlorophenoxyacetic acid (2, 4- D), 6- benzylaminopurine (6- BA), 6- furfurylaminopurine (6- FA), indolebutyric acid (IBA), ABT1 (commercial name) and zeatin (ZT) as additional compositions were used for inducing, differentiating and rooting tests. Induced results showed that 2, 4- D had better promoting effect ; the combination of 0. 5mg/L (2, 4- D) and 2 mg/L (6- BA) had 90 percent of induction, but IAA had no effect. NAA and 6- BA showed a better differentiation effect; the combination of 1 mg/L NAA+ 4 mg/L (6- BA) 42 mg/L ZT had a high differentiation percentage, up to 81. 1 %, but 6- FA had not apparent effect on differentiation. The rooted results showed that these three culture media, MS+0. 5 mg/L NAA+0. 5.mg/L (6-FA), MS+0. 4 mg/L NAA+2 mg/L IBA, MS+0. 2 mg/L NAA+0. 1 mg/L (6- FA) +ABT1, all emerged roots, the rooted ratio were 70% above. The roots showed thick as if active carbon was added to the culture medium, the roots emerged were thin and tenacious.