| GPC-RI-MALLS技术测定羟乙基淀粉的分子量及分子量分布 |
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| 引用本文： | 吴扬兰,王远亮,徐世荣,杨亚联,王喜云.GPC-RI-MALLS技术测定羟乙基淀粉的分子量及分子量分布[J].重庆大学学报(自然科学版),2007,30(7):121-123129. |
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| 作者姓名： | 吴扬兰 王远亮 徐世荣 杨亚联 王喜云 |
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| 作者单位： | 重庆大学生物工程学院,重庆,400030;重庆大学机械传动国家重点实验,重庆,400030 |
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| 基金项目： | 国家自然科学基金
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重庆大学校科研和教改项目 |
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| 摘 要： | 羟乙基淀粉是一种优良的血浆代用品,作者介绍了用凝胶渗透色谱(GPC)-示差检测(RI)-多角度激光光散射(MALLS)联用技术测试羟乙基淀粉的分子量和分子量分布的实验技术,给出了测试折光指数增量dn/d c值、分子量和分子量分布实验步骤和方法,并对其分析测试过程中的关键实验技术及实验结果进行了详细的讨论.该方法以CH3COOH/CH3COONa溶液作为流动相,实验获得了满意的结果,可作为羟乙基淀粉分子量和分子量分布测试的常规分析方法.
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| 关 键 词： | 激光光散射法 GPC 羟乙基淀粉 代血浆产品 分子量 分子量分布 测定 |
| 文章编号： | 1000-582X（2007）07-0121-03 |
| 修稿时间： | 2007-03-22 |
Determination of Molecular Weight and Distribution of Hyroxyethl Starch (HES) by Means of GPC- RI- MALLS Technique |
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| WU Yang-lan,WANG Yuan-liang,XU Shi-rong,YANG Ya-lian,WANG Xi-yun.Determination of Molecular Weight and Distribution of Hyroxyethl Starch (HES) by Means of GPC- RI- MALLS Technique[J].Journal of Chongqing University(Natural Science Edition),2007,30(7):121-123129. |
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| Authors: | WU Yang-lan WANG Yuan-liang XU Shi-rong YANG Ya-lian WANG Xi-yun |
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| Institution: | 1. College of Bioengineering; 2. State KeyLaboratory of Mechanical Transmission, Chongqing University, Chongqing 400030, China |
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| Abstract: | The author introduces a method and experimental procedures that determine dn/dc,molecular weight and its distribution of the hyroxyethyl starch(HES) by means of Gel Permeation Chromatography-Refractive index-Multi-angle laser light scattering detection(GPC-RI-MALLS)techniques.The key experimental technique and results are discussed.The method takes CH3COOH/CH3COONa as mobile phase and solvent.The experiment gets satisfactory results.It can be used as a routine method to determine the hyroxyethyl starch(HES) molecular weight and its distribution. |
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| Keywords: | laser light scattering method the hyroxyethyl starch(HES) plasma substitutes GPC molecular weight molecular mass distribution determination |
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