| Autophagy Plays a Potential Role in the Process of Sea Cucumber Body Wall ＂Melting＂ Induced by UV Irradiation |
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| 作者姓名： | ZHU Beiwei ;ZHENG Jie ;ZHANG Zongshen ;DONG Xiuping ;ZHAO Lulu ;Tada Mikiro |
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| 作者单位： | [1]College of Biology and Food Engineering, Dalian PolytechnicUniversity, Dalian 116034, Liaoning ,China; [2]Department of Biological Resources Chemistry, Faculty ofAgriculture, Okayama University, Okayama 700-8530, Japan |
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| 基金项目： | Supported by the National Natural Science Foundation of China（30571449）, the National Basic Research Program of China （2006CB708210） and the Chinese International Corporation Project （2006DFA32580） Acknowledgements： We thank Prof. Murata Yoshiyuki （Department of Biological Resources Chemistry, Faculty of Agriculture, Okayama University） for giving us useful suggestions and thank Mr. Gao Chuanzhou, Ms Lu Guangyan and Qu Shuxian （Dalian Medical University） for their technical assistances in TEM sampling and sections observation. |
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| 摘 要： | The changes of tissue appearances and structures in the process of UV-induced ＂melting＂ for sea cucumber （Stichopus japonicus） body wall were studied. And the localization and determination of acid phosphatase （ACP）, Cathepsin B and Cathepsin L activities were also investigated. The results show that the connective tissue was damaged with many hollows emerging and the regular collagen bundles were broken apart into irregular fragments. Margination of condensed chromatin at the nuclear membrane was observed. Both Golgi＇s body and endoplasmic reticulure swelled, curled, and eventually double- or multi-lamellar vesicles were formed. A number of autophagic vesicles distributed in all through the whole cytoplasm. ACP becomes more active after UV irradiation. The activities of cathepsin B and cathepsin L increased in UV-treated sea cucumbers and both achieved their maximum under certain conditions, It indicates that autophagy plays a potential role in the ＂melting＂ process for sea cucumber body wall induced by UV irradiation.
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| 关 键 词： | 海黄瓜 磷酸酶 组织蛋白酶 细胞生物学 |
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