Studies on the reconstitution ofP. clarkii blue carotenoprotein from its isolated subunits |
| |
Authors: | R Gomez J M Macarulla A M Gárate P G Barbón J C G Milicua |
| |
Institution: | (1) Department of Biochemistry, University of the Basque Country, P.O. Box 644, Bilbao, (Spain) |
| |
Abstract: | Summary A blue carotenoid-protein complex ( max 635 nm) was extracted and purified from the carapace of the crayfishProcambarus clarkii. The complex was further liberated from astaxanthin, its prostetic group, causing dissociation into apoprotein subunits. Reconstitution of the complex from the various sub-units (isolated by chromatofocusing) plus astaxanthin was attempted. Apoprotein-size pigments of rose-purple color ( max 545 nm) were obtained. It was found that both monomers are required in order to a blue complex fairly similar in structure ot the native one. However, the native conformation was not completely recovered, as indicated by some differences in the UV spectrum. |
| |
Keywords: | Carotenoproteins protein reconstitution astaxanthin |
本文献已被 SpringerLink 等数据库收录! |