三尖杉试管苗生根诱导的初步研究 |
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引用本文: | 蔡月琴,;陈羡德,;陈礼光,;林镇斌,;蔡长福,;郑郁善.三尖杉试管苗生根诱导的初步研究[J].漳州师院学报,2008(4):113-116. |
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作者姓名: | 蔡月琴 ;陈羡德 ;陈礼光 ;林镇斌 ;蔡长福 ;郑郁善 |
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作者单位: | [1]漳州师范学院生物科学与技术系,福建漳州363000; [2]福建农林大学林学院,福建福州350002; [3]三明市林业局,福建三明365001 |
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基金项目: | 福建省林木种苗科技攻关计划项目(021812). |
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摘 要: | 以三尖杉茎段诱导的腋芽苗为外植体进行生根诱导研究.结果表明,改良WH附加一定浓度的IBA能诱导三尖杉试管苗生根,NAA单独处理时未能诱导三尖杉不定根的发生;IBA、NAA共同使用时生根诱导效果比IBA单独使用时好,其中以改良WH_+mA3.0mg/L+NAA1.0mg/L培养基组合生根诱导效果较好,生根率为60.7%.
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关 键 词: | 三尖杉 试管苗 离体培养 生根诱导 |
A Preliminary Study on Rooting Induction of Cephalotaxusfortunei Hook.f. |
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Institution: | CAI Yue-qin, CHEN Xian-de, CHEN Li-guang, LIN Zhen-bin, CAI Chang-fu, ZHENG Yu-shan(1. Department of Biological Science and Technology, Zhangzhou Normal University, Zhangzhou, Fujian 363000, China; 2. College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; 3. Forestry Bureau of Sanming City, Sanming, Fujian 365001, China) |
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Abstract: | Rooting induction for C. fortunei was studied using regenerated plantlets as explants. The results indicated that only the mend-WH adding a certain concentration of IBA could induce rooting, but the use of NAA separately could not. And the effect of rooting in the mend-WH adding IBA and NAA within a certain concentration was better than in the mend-WH adding IBA separately. The mend-WH adding IBA 3.0 mg/L and NAA 1.0 mg/L was perfectly suitable to the root induction, in which the rate of rooting induction was 60.7 %. |
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Keywords: | Cephalotaxusfortunei regenerated plantlets culture in vitro rooting induction |
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