复方山楂煎剂对非酒精性脂肪肝大鼠模型的影响

目的 研究复方山楂煎剂对非酒精性脂肪肝的治疗作用及其作用机制。方法 将60只Wistar大鼠随机分 为空白对照组、模型对照组、复方山楂煎剂(低剂量14. 85 g生药/kg、中剂量29.7 g生药/kg、高剂量59.4 g生药/ kg)组，脂必妥对照组，除空白对照组外其余各组采用高脂饲料喂养大鼠8周，造模后空白对照组、模型对照组灌胃给予蒸馏水，其余各组灌胃给予相应药物，治疗6周。实验结束后，检测各组大鼠血清丙二醛(MDA)、超氧化物歧 化酶(SOD)、谷丙氨酸转氨酶(ALT)、谷草转氨酶(AST)以及肝组织中的甘油三酯(TG)和肝体比，并采用HE染色 观察肝组织脂肪变性程度，采用免疫组化法检测肝组织中肝细胞生长因子(HGF)及结缔组织生长因子(CTGF)的 表达。结果 复方山楂煎剂可显著降低脂肪肝模型大鼠肝体比、血清MDA、ALT以及肝脏TG含量(P<0.05或P< 0.01)和结缔组织生长因子(CTGF)在脂肪肝模型大鼠肝组织中的表达(P<0.05)。复方山楂煎剂中剂量组、高剂量组可显著降低脂肪肝模型大鼠血清AST含量(P<0. 05或P<0. 01)、显著提高脂肪肝模型大鼠血清SOD的活性 (P<0. 05或P<0.01)和显著降低肝细胞生长因子(HGF)在脂肪肝模型大鼠肝组织中的表达(P <0.05)。复方山楂 煎剂可显著降低高脂饮食诱发的肝细胞脂肪变性程度。结论复方山楂煎剂可有效地用于非酒精性脂肪肝的治疗。

Effect of Shanzha Decoction on Nonalcoholic Fatty Liver in Rats

Objective To observe the effect of Shanzha decoction on nonalcoholic fatty liver model in rats. Method Sixty Wistar rats were randomly divided into control group, model control group and Shanzha decoction (lower 14. 85 g shengyao/kg, middle 29. 7 g shengyao/kg, high 59. 4 g shengyao/kg dose group) . In the group, except for the blank control group, the rats were fed with high-fat diet for 8 weeks. The blank control group and the model control group were given distilled water by gavage, and the other groups were given the corresponding drugs for 6 weeks. At the end of the experiment, serum malondialdehyde ( MDA ) , superoxide dismutase ( SOD ), alanine aminotransferase ( ALT) , aspartate aminotransferase ( AST) , and triglycerides in liver tissue ( TG ) were measured in each group. The expression of hepatocyte growth factor and connective tissue growth factor in liver tissue was detected by immunohistochemistry. Result Shanzha Decoction significantly reduced liver index, serum MDA, ALT, AST and liver TG content in rats with fatty liver ( P <0. 05 or P <0. 01) , and significantly increased serum SOD activity in rats with fatty liver ( P <0. 05 or P<0. 01) , Shanqi decoction group can significantly reduce the expression of hepatocyte growth factor and connective tissue growth factor in liver tissue of rats with fatty liver ( P<0. 05) . Conclusion Shanzha decoction can be effectively used for the treatment of nonalcoholic fatty liver.