妊娠期高血压对大鼠肝脏Ⅰ型脱碘酶及甲状腺激素的影响研究

目的探讨妊娠期高血压疾病对大鼠肝脏Ⅰ型脱碘酶以及甲状腺激素的影响。方法将20只孕鼠分为正常妊娠对照组(n=10)和妊娠高血压模型组(n=10)。于妊娠第13天,模型组大鼠皮下注射L-NAME至妊娠第21天,复制妊娠高血压疾病;对照组皮下注射等量生理盐水,起止时间同模型组一致。采集两组大鼠肝脏和血液,进行肝脏DIO-ⅠmRNA表达量和血液甲状腺激素水平检测。结果与对照组比较,模型组大鼠肝脏DIO-ⅠmRNA表达量显著下降(P <0. 05),血液中TT3、FT3浓度显著降低(P <0. 05)。结论妊娠期高血压疾病可降低孕鼠肝脏DIO-Ⅰ基因表达水平,进而导致血液TT3、FT3含量下降。

The effect of HDCP on Type I lodothyronine Deiodinases and Thyroid Hormone

To investigate the effect of HDCP on DIO- I and thyroid hormone. Method Twenty pregnant rats were divided into control group ( n = 10 ) and model group ( n = 10 ) . On the 13 th day of gestation , rats in model group were received L-NAPE by subcutaneous injection until 21th day of gestation , HDCP model was reproduced. Control group received saline by the same ways and periods as model group. To get the livers ample and serum, the expression of DIO- I mRNA in liver and thyroid hormone level in serum were detected. Result Compared with control group, the expression of DIO- I mRNA in liver of model group was significantly decreased (P<0. 05 ) , and the TT3 , FT3 level in serum of model group were notably dropped ( P<0. 05 ) . Conclusion HDCP can result in the reduction of the expression of DIO-I mRNA in rat liver , and then the serum level of TT3 and FT3 are both dropped .