腹主动脉缩窄法建立大鼠心衰模型的研究

目的 通过研究不同程度缩窄腹主动脉对Wistar大鼠心脏功能的影响，建立大鼠心衰模型。方法 将Wistar 大鼠随机分成3组,A组为假手术组,B组缩窄腹主动脉直径至0. 45 mm,C组缩窄腹主动脉直径至0. 7 mm,术后观察大鼠生存情况、精神状态等一般情况，在4周和8周分别应用超声心动图检测舒张末期室间隔厚度（IVSd）、舒张 末期左室后壁厚度（LVPWd）、左室舒张末期内径（LVEDd）、左室收缩末期内径（LVEDs）和射血分数（LVEF）,并取 标本测量左心室质量指数LVWI、心/体比、肺/体比，评价心功能的变化。结果 术后A组存活率为100%、B组存活率为55%、C组存活率为70%>；4周时B组IVSD、LVPWd、LVEDd、LVEDs、LVEF与A组和C组均有显著差异（P 0. 05）。8周时B、C两 组IVSD、LVPWd、LVEDd、LVEDs、LVEF与A组相比差异显著（P <0. 05） ；8周时LVWI、心/体比、肺/体比B组和C 组分别与A组相比均有极显著差异（P<0.01），C组与B组相比LVWI、心/体比有显著差异（P <0. 05），肺/体比差 异极显著（P<0.01）。结论 腹主动脉缩窄至直径0. 45 mm比缩窄至0. 7 mm心衰成模所需时间短，成模效率高， 是建立大鼠心衰模型的理想方法。

Research of the Establishment of Rat Model of Heart Failure byAbdominal Arterial Constriction

Objective To establish rat model of heart failure by constriction of abdominal aorta, and to compare the effects of abdominal aorta narrowing with different diameters on hemodynamics and pathophysiology in rats. Method Wistar rats were randomly divided into three groups : sham-operated group ( group A ) ; narrowed abdominal aorta diameter up to 0. 45 mm ( group B ) ; narrowed abdominal aorta diameter up to 0. 7 mm ( group C ) ; observed the survival condition and mental state of the rats after the operation, at 4 and 8 weeks detected the interventricular septal depth ( IVSd )、left ventricular posterior walldepth ( LVPWd )、left ventricular end diastolic diameter (LVEDd )、left ventricular end systolic diameter ( LVEDs )、left ventricular ejection fraction ( LVEF ) by echocardiography, and left ventricular mass index( LVMI)、heart/body ratio、lung/body ratio were investigated after 8 weeks. Result The postoperative survival rate in group A is 100% , in group B is 55%, in group C is 70 % ; After 4 weeks, IVSd, LVPWd, LVEDs, LVEDd and EF in group A were significantly different from those in group B and C ( P 0. 05 ) . While after 8 weeks, IVSD、LVPWd、LVEDd、LVEDs、 LVEF in group B and C both had more significant changes compared with those in group A ( P< 0. 05 ) ;at 8 weeks LVMI、heart/body ratio、lung/body ratio were increased markedly in both group B and group C compared with group A ( P<0. 01) , meanwhile compared with group C , LVWI and heart/body ratio were much higher than those in group B ( P <0. 05 ) , and lung/body ratio increased more obviously ( P <0.01). Conclusion Coarctation of abdominal aorta diameter up to 0. 45 mm required shorter time, and has more efficient than narrowed up to 0. 7 mm in heart failure model establishment, it is an ideal method to establish rat model of heart failure.