| 一株产普鲁兰酶细菌的分离鉴定及其发酵条件优化 |
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| 引用本文： | 王明道,郭双,张雨杭,孙利鹏,时延光,邱立友. 一株产普鲁兰酶细菌的分离鉴定及其发酵条件优化[J]. 信阳师范学院学报(自然科学版), 2014, 0(4): 569-573 |
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| 作者姓名： | 王明道 郭双 张雨杭 孙利鹏 时延光 邱立友 |
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| 作者单位： | 1. 河南农业大学 农业部农业微生物酶工程重点实验室,河南 郑州,450002
2. 河南仰韶生化工程有限公司,河南 渑池,472400 |
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| 基金项目： | 科技部“十二五”农村领域科技计划项目(2013AA102101-2)；河南省重点科技攻关计划项目 |
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| 摘 要： | 从淀粉加工厂附近土壤中筛选得到一株普鲁兰酶高产菌株,编号为Z-13,其初始酶活达到5.7 U/mL.通过对此菌株的16S rDNA比对,以及生理生化鉴定,鉴定此菌株为克雷伯氏菌(Klebsiella variicola),通过发酵条件优化,确定最佳发酵产酶条件为:玉米淀粉1.5%,蛋白胨2.0%,KH2PO40.05%,MgSO4·7H2O 0.01%.装液量为70 L/250 L,培养基最初pH为6.0,发酵温度30℃,摇床转速200 r/min,发酵时间48 h,优化后菌株产普鲁兰酶的酶活高达67.8 U/mL,是优化前菌株产普鲁兰酶活性的11.89倍.
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| 关 键 词： | 普鲁兰酶 筛选 鉴定 产酶条件优化 |
Isolation,Identification of A Bacterial Strain Producing Pullulanase and Optimization of its Fermentation Conditions |
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| Wang Mingdao,Guo Shuang,Zhang Yuhang,Sun Lipeng,Shi Yanguang,Qiu Liyou. Isolation,Identification of A Bacterial Strain Producing Pullulanase and Optimization of its Fermentation Conditions[J]. Journal of Xinyang Teachers College(Natural Science Edition), 2014, 0(4): 569-573 |
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| Authors: | Wang Mingdao Guo Shuang Zhang Yuhang Sun Lipeng Shi Yanguang Qiu Liyou |
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| Affiliation: | Wang Mingdao;Guo Shuang;Zhang Yuhang;Sun Lipeng;Shi Yanguang;Qiu Liyou;Key Laboratory of Enzyme Engineering of Agricultural Microbiology,Ministry of Agriculture,Henan Agricultural University;Henan Yangshao Bio-products Co. LTD; |
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| Abstract: | A high-producing strain of pullulanase Z-13 was isolated from the soil near a starch-processing factory.The initial activity was 5. 7 U /mL. Based on 16 S rDNA sequence homology analysis,Z-13 was identified as klebsiella variicola,with its physiological and biochemistry characters. The optimum fermentation condition for Z-13 was determined as follows: corn starch 1. 5%,peptone 2. 0%,KH2PO40. 05%,MgSO4·7H2O 0. 01%. When cultured at optimum fermenting conditions(studied as follows: the content of flask is 70 L /250 L,the inoculation quantity was 8%,cultured at pH 6. 0,30 ℃,200 r /min for 48 hours),the activity of pullulanase produced by Z-13 reached the peak at67. 8 U /mL,which was 11. 89 times as the initial activity. |
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| Keywords: | pullulanase isolation identification optimization of fermentation conditions |
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