桑黄菌质多糖的固态发酵及其抗氧化作用

实验采用固态发酵的方法,将经液态发酵产生的桑黄菌球接种到固态培养基中继续发酵,寻找最佳培养基并对其进行优化,确定最佳培养基配方。提取桑黄菌质多糖,灌胃给予环磷酰胺(CP)创伤小鼠,观察其对小鼠血清和肝组织中超氧化物歧化酶(SOD)活性和丙二醛(MDA)质量分数的影响。抗氧化实验表明,桑黄菌质多糖可明显提高小鼠血清和肝组织SOD活性,降低MDA质量分数,具有抗脂质过氧化的作用。

Solid fermentation of polysaccharide from Phellinus igniarius and its effects on SOD and MDA in blood of mice

The method of solid fermentation was used,and the germ ball from liquid fermentation was inoculated to the solid optimal medium to continue fermentation in order to find and optimize the solid optimal medium.Then the optimal medium of polysaccharide from Phellinus igniarius produced by the way of solid fermention was selected.At the same time,the effects of polysaccharide from Phellinus igniarius on superoxide dismutase(SOD) and malondialdehyde(MDA) in blood of the CP mouse were observed.The determined optimal medium is as follows:bran 100 g,glucose 1.5 g,cream of beef 1.5 g,MnSO4 0.05 g,water 100 mL.The results of the antioxidation experiment indicate that polysaccharide from Phellinus igniarius obviously develops the activity of SOD and reduces the content of MDA and it has the effect of antioxidation.