低频电刺激对脏器中激素含量的影响

摘要: 目的探索低频电刺激对中国树鼩死亡后脏器中激素水平的影响。方法给予中国树鼩低频电刺激，分别于死亡后0 h、3 h、6 h、12 h、18 h、24 h、36 h 和72 h 取甲状腺、肝脏、脾脏，用放射免疫法测定内皮素( endothelin，ET) 、心钠素( atrial natriuretic factor，ANF) 、血栓素( thromboxane，TX) 水平; 剥取死后0 h 中国树鼩中脑腹侧背盖区( ventral tegmental area，VTA) 检测c-fos 表达。结果电刺激后，中国树鼩尸体脏器中内皮素、心钠素、血栓素水平比对照组显著升高，其含量随死亡后时间的延长而降低，且呈现一定的趋势; VTA c-fos 表达明显增强。结论低频电刺激能引起中国树鼩脏器中激素的合成、释放和脑组织c-fos 表达。

Effect of Low Frequency Electrical Stimulation on Hormone Levels

Abstract: Objective To study the effect of low frequency electrical stimulation on hormone levels in organs of Chinese tree shrews after death． Method Chinese tree shrews were given low frequency electrical stimulation，0 h，3 h，6 h，12 h，18 h，24 h and 36 h and 72 h after death，thyroid，liver，spleen were taken，respectively，and levels of endothelin ( ET) ，atrial natriuretic factor ( ANF) ，thromboxane ( TX) were determined by ＲIA method; c-fos expression of midbrain ventral tegmental area ( VTA) of Chinese tree shrews at 0 h after the death was measured． Ｒesult After electrical stimulation，ET，ANF，TX level in body organs and c-fos expression in VTA of Chinese tree shrews were significantly increased than the control group， the contents were decreased with the extension of time after death． Conclusion Low frequency electrical stimulation can induce the synthesis and release of the hormone in organs and c-fos expression of brain tissue in Chinese tree shrews．